Application of nano zinc in controlling of multi drug resistant pathogenic Escherichia coli | ||||
| Mansoura Journal of Biology | ||||
| Volume 56, Issue 3, September 2022, Page 31-41 PDF (1.05 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjb.2022.450625 | ||||
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| Authors | ||||
| Elshafey, A.M* 1; Abou El-khier .N.T2; Abdel-Fattah, G.M1 | ||||
| 1Department of Botany, Faculty of Science, Mansoura University, Egypt | ||||
| 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Egypt. | ||||
| Abstract | ||||
| Multi drug resistance (MDR) is becoming a global problem for public health which threatens the lives of hospitalized individuals as well as increases health care costs and long-time treatment. Further spread of the antimicrobial resistance occurs owing to misapplication of antimicrobial medications and hurries the development of drug-resistant strains. Zinc oxide nano particles (ZnO NPs) are used as alternative antibacterial agent for controlling MDR pathogenic E. coli. About 75 clinical E. coli isolates were collected from patients admitted to Mansoura University Hospitals and Mansoura Insurance Hospital. Antibiotic susceptibility pattern was tested by the disc diffusion method. We selected only 40 MDR isolates. All 40 MDR isolates were tested for the effect of different concentrations of ZnO NPs alone as an antibacterial agent and the combined effect of antibiotics and ZnO NPs as an antibacterial agent by the agar well diffusion method. E. coli isolates showed the highest resistance to azteronam, cefipime, cefotaxime, amoxicillin clavulanic acid, ciprofloxacin and cefaclor with (100%) followed by piperacillin tazobactam with (95%), followed by norfloxacin, tetracycline and sulphamethoxazole trimethoprim with (92.5%), Of all concentrations of ZnO NPs, the stock (4.176g/l) was the most active one with inhibition zones diameter ranged between (14-26mm) and the second concentration (1.392 g/l) caused inhibition zones diameter ranged between (14-24mm), followed by (0.464, 0.154, 0.051, 0.017, 0.0057 g/l) respectively. The antibacterial activity of antibiotics such as meropenem, piperacillin tazobactam, cefipime, amoxicillin clavulanic acid, ciprofloxacin, gentamycin, amikacin, azteronam, nitrofurantoin, sulphamethoxazole trimethoprim, cefaclor, tetracyclin and norfloxacin respectively against E. coli was increased considerably in the presence of ZnO NPs while the antibacterial activity of cefotaxime was not increased considerably in the presence of ZnO NPs. | ||||
| Keywords | ||||
| Multi drug resistance (MDR); Escherichia coli (E. coli) and Zinc oxide nanoparticles (ZnO NPs) | ||||
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