Isolation And Study of The Molecular Characteristics of Most Gram-Negative Bacteria from Cases of Genital Tract Infections in Cows | ||
| Assiut Veterinary Medical Journal | ||
| Articles in Press, Accepted Manuscript, Available Online from 20 October 2025 | ||
| Document Type: Research article | ||
| DOI: 10.21608/avmj.2025.369724.1640 | ||
| Authors | ||
| balsam almahmood* 1; Frah Nooh2 | ||
| 1Microbiology, college of veterinary science/university of Mosul | ||
| 2Microbiology, college of veterinary science/ university of Mosul | ||
| Abstract | ||
| Bacterial infections of the genital tract are among the most important problems facing livestock herds, leading to economic losses, in addition to infertility and low production. Our research was conducted with the aim of isolating and molecularly studying most crucial Gram-negative microorganisms that are responsible for genital tract infections in cows, investigating these bacteria's susceptibility to the most significant antibiotics, and identifying the most important virulence factors. Escherichia coli gave the highest isolation rate of 44%, followed by Proteus mirabilis at 7.4%, and the lowest percentage was for Klebsiella spp, Serratia ficaria and Enterobacter spp 1.5%. Through the study, E. coli bacteria were the most isolated in cases of metritis endometritis, and vaginitis. It was also isolated at a high rate in cases of retained placenta and dystocia, which is considered a predisposing factor for reproductive system infections with percentage 33.3&20% respectively. E. coli has shown absolute resistance to the antibiotics Imipenem, Tetracycline, and Cefixime. while Proteus mirabilis showed absolute resistance to Azithromycin, Tetracycline and Nitrofurantoin. Virulence factors Stx1, Stx2, and Uid were present in all E. coli. While all the isolates of Proteus mirabilis contained the virulence genes zapA and ureC. Bacteria are important causes of infertility and abortion, which cause production losses and poor meat production. Therefore, it is important to identify these causes and study the most important effects of virulence factors that they cause on the genital tract in cows. | ||
| Keywords | ||
| Genital tract infection; E.coli; Proteus spp; PCR | ||
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