Monitoring of resistance in four field strains of Culex pipiens using bioassay and agar gel electrophoresis techniques | ||
| Journal of Pest Control and Environmental Sciences | ||
| Volume 4, Issue 1, June 1992, Pages 1-13 PDF (4.13 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jpces.1992.462129 | ||
| Authors | ||
| M. Mourad1; Fathia I. Moustafa2; M. S. Shawir* 2 | ||
| 1Agricultural Research Center. CenteraI Pesticide Lab. Sabahia. Alexandria | ||
| 2Division of Pesticides, Plant Protection Department Faculty of Agriculture. University of Alexandria. Egypt. | ||
| Abstract | ||
| A survey of the susceptibility level in larvae of Culex pipiens conducted at four areas representing the Alexandria governorate namely Abou-Ker, El-Amria, Moharram-Bey and Sidi-Bishr. The standard bioassay technique of the WHO was used in larvae treatments to follow the development of resistance phenomenon. The susceptibility levels for ten - insecticides representing the main groups of insecticides are fluctuated around increasing or decreasing with no sing of resistance. The susceptibility level showed that two out of the four localities revealed tolerance to malathion and the most tolerant population was Sidi-Bisher followed with Abou-Ker. Likewise, tolerance to fenitrothion. carbaryl and methomyl was observed in two strains (Moharram-Be and Sidi-Bisher). Sidi-Bisher population is considered the most tolerant population either for organophosphates or carbamates. A Test for the determination of the esterase responsible to organophosphate resistance. in a single mosquito using agar gel electrophoresis technique showed a close correlation between the frequency of this esterase and level of tolerance in the tested strains. | ||
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