A Critical Review of High-Performance Liquid Chromatography in the Analysis of Water-Soluble Vitamins | ||
| Sphinx Journal of Pharmaceutical and Medical Sciences | ||
| Volume 10, Issue 1, October 2025, Pages 49-75 PDF (1.62 M) | ||
| Document Type: Review articles | ||
| DOI: 10.21608/sjpms.2025.366755.1041 | ||
| Authors | ||
| Pakinaz Khashaba* 1, 2; Sally A. El Zohny3; Moamen Ahmed4; Kirolos Samy4; Bianca Weal4; Marina Musad4; Marleen Kamal4; Emam Abdelhay4; Michael Zaky4; Yousif Ashraf4 | ||
| 1Department of Pharmaceutical chemistry, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt | ||
| 2Department of Pharmaceutical analytical chemistry, Faculty of Pharmacy, Assiut University, Assiut, Egypt | ||
| 3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sphinx University, New Assiut, Egypt | ||
| 4Faculty of Pharmacy, Sphinx University, New Assiut, Egypt | ||
| Abstract | ||
| This review presents an in-depth analysis of the advancements in High-Performance Liquid Chromatography (HPLC) for detecting and measuring water-soluble vitamins, such as thiamine (B1), riboflavin (B2), pyridoxal (B6), and cobalamin (B12), from 2000 to 2024. These vitamins play essential roles in metabolic functions, energy synthesis, and antioxidant defense mechanisms, making their accurate quantification necessary for food and pharmaceutical industries. Their instability under environmental conditions, including heat, light, and oxygen exposure—further emphasizes the demand for precise detection methods. HPLC has become the preferred analytical technique due to its high sensitivity, selectivity, and ability to analyze complex food compositions. This review highlights significant improvements in chromatographic separation techniques and detection methods that have enhanced accuracy, reproducibility, and reliability in vitamin analysis. Innovations in detector technologies have minimized matrix interferences, leading to more dependable results. Ongoing improvements in HPLC are essential to meet the increasing demand for precise vitamin quantification. This study underscores HPLC's evolution as a critical tool in ensuring food quality and safety | ||
| Keywords | ||
| Chromatographic separation; Food and pharmaceutical analysis; High-Performance Liquid Chromatography (HPLC); Vitamin quantification; Water-soluble vitamins | ||
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