Screening for Blue-green Pigment Production by Pseudomonas Isolates | ||
| Journal of Agricultural Chemistry and Biotechnology | ||
| Volume 16, Issue 10, October 2025, Pages 161-165 PDF (413.79 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/jacb.2025.435062.1127 | ||
| Authors | ||
| Dina S. M. A. Shaaban1; M. A. E. Selim1; W. I. A. Saber2; A. M. M. Elattaapy* 1 | ||
| 1Microbiology Dept., Fac. of Agric., Mansoura Univ., Egypt | ||
| 2Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt | ||
| Abstract | ||
| Eight isolates belonging to the genus Pseudomonas were isolated from various environmental sources, including soil, water, and food material, and tested for blue-green pigment production. All selected isolates exhibited good production of the blue-green pigment, with three isolates showing high levels of pigment production, yielding 2.13, 3.47, and 5.74 µg/mL when cultured in nutrient broth medium supplemented with 1% waste oil. In an attempt to select an appropriate medium for blue-green pigment production, six different media were tested with all isolates. The Obtained data indicate a significant variation in microbial pigment production among the different culture media. The data revealed that King’s medium was the most effective for pigment production compared to nutrient broth and Bushnell Haas media with all tested Pseudomonas isolates. On the other hand, the results demonstrated that the supplementation of nutrient broth and Bushnell Haas media enhanced pigment production. Nutrient broth (Oxoid) showed high productivity with all isolates when supplemented with waste oil or glycerol. Maximum pigment production was achieved by isolate W4, yielding 4.98 µg/mL when cultured in waste oil-supplemented nutrient broth. | ||
| Keywords | ||
| Blue-green pigment; Waste-oil; Pseudomonas | ||
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