MOLECULAR DETECTION OF SOME VIRULENCE GENES OF E. COLI ISOLATED FROM CALF DIARRHEA IN SIWA OASIS, EGYPT | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 84, Issue 1, April 2024, Pages 29-45 PDF (571.84 K) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| Pathogenic Escherichia coli (E. coli) is the bacteria that causes diarrhea in calves most often, especially in young calves So, this study showed the prevalence and antibiogram pattern of E. coli found in calves with diarrhea in different areas in SIWA oasis ,Egypt. In addition, the polymerase chain reaction (PCR) was used to look at six genes that code for virulence (IutA, iss, fimH, papC, adrA and TSH). One hundred seventy calves were checked to see if they had E. coli. From samples of 170 calves with diarrhea, 86 (50, 5%) were found to be E. coli infected isoletes were studied were very sensitive to amoxicillin/clavulanic acid, polymixin, enrofloxacin, and ciprofloxacin.On the other hand, they were very resistant to streptomycin, gentamycin, tetracycline, sulfamethoxazole/trimethoprim, chloramphenicol and neomycin. The most common virulence genes found in all of the tested strains were iss, fimH and iutA. This was followed by (papC) gene (76%) (AdrA) gene (35%) while TSH (9%). Our research showed that colibacillosis in calves is caused by a strain that is resistant to multiple antibiotics and has virulence genes. This is important for coming up with new ways to control colibacillosis in calves. . | ||
| Keywords | ||
| Bovine; Escherichia coli; Virulence Gene; Antibiogram; Siwa Oasis | ||
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