CHARACTERIZATION OF ANTIMICROBIAL RESISTANT ESCHERICHIA COLI ISOLATED FROM MASTITIC COWS AND BUFFALOES | ||
| Journal of the Egyptian Veterinary Medical Association | ||
| Volume 84, Issue 1, April 2024, Pages 57-76 PDF (649.47 K) | ||
| Document Type: Original Research Articles | ||
| Abstract | ||
| Mastitis is one of the most costly production diseases in the dairy industry that is caused by a wide array of microorganisms. E. coli is a common cause of bovine mastitis even in well-managed herds and therefore, E. coli was targeted in this study. Out of 200 milk samples from cows and buffaloes, 76 resulted in isolation of E. coli (38%). Of the recovered isolates, 71were obtained from 160 mastitic cows’ milk samples and 5 were from 40 mastitic buffaloes’ at Giza governorate of Egypt. E. coli isolates were isolated, identified biochemically and confirmed at the molecular level. According to serotyping, the detected E. coli serotypes of E. coli from cows’ milk were O148, O125, O166, O158, O146, O126, O27, O26, O111, O1, O18, O119, O86a, O114, O78, O169, O25, O168, and O157 while serotypes detected from buffaloes’ milk were O55, O125, O86a and O124 from buffalo mastitic milk. Antimicrobial susceptibility test was carried out on the isolates using the disk diffusion method to investigate the potential multi drug resistance (MDR) among the isolates. All isolates were resistant to one or more antibiotics. The recovered E. coli strains were also screened by polymerase chain reaction to detect certain antibiotic resistance genes (namely; bla Tem, bla CTX-M, qnr, and Kan genes) as well as virulence genes (stx2 And eaeA). | ||
| Keywords | ||
| Mastitis; E. coli; Serotypes; MDR; PCR; Antimicrobial Resistance Genes; Virulence Genes | ||
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