Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin
Abozaid, O., EL- Elsonbaty, S., Gad, A. (2018). Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin. EKB Journal Management System, 35(2), 547-558. doi: 10.21608/bvmj.2018.111627
Omayma Abozaid; Sawsan EL- Elsonbaty; Asmaa Gad. "Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin". EKB Journal Management System, 35, 2, 2018, 547-558. doi: 10.21608/bvmj.2018.111627
Abozaid, O., EL- Elsonbaty, S., Gad, A. (2018). 'Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin', EKB Journal Management System, 35(2), pp. 547-558. doi: 10.21608/bvmj.2018.111627
Abozaid, O., EL- Elsonbaty, S., Gad, A. Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin. EKB Journal Management System, 2018; 35(2): 547-558. doi: 10.21608/bvmj.2018.111627

Irradiated β - Glucan attenuates diethyl nitrosamine induced hepatocarcino-genesis through regulation of CD4,CD8 and β – Catenin

Benha Veterinary Medical Journal
Article 46, Volume 35, Issue 2, December 2018, Page 547-558  XML PDF (559.59 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.111627
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Authors
Omayma Abozaid1; Sawsan EL- Elsonbaty2; Asmaa Gad1
1Department of Biochemistry, Faculty of Veterinary Medicine, Benha University, Egypt;
2Radiation Microbiology Department, National Center for Radiation Research and Technology, Atomic Energy Authority, Cairo, Egypt
Abstract
β-glucans are heterogeneous group of natural polysaccharides mostly investigated for their immunological effects. The objective of this study was to evaluate the efficiency of irradiated β-glucan on N-nitrosodiethylamine induced hepatocarcinogenesis in rats.β-glucan powder was exposed to 50 KGY of gamma radiation for experimental study, healthy male rats were divided in five groups. Group 1: did not receive any treatment. Group 2: hepatic cancer group rats administrated with DEN orally at a dose of (20mg/kg b.wt/day) for six weeks. Group 3: Irradiated β-glucan group rats were orally administrated with Iβ- glucan for six weeks at a dose of 65 mg/kg body weight/day. Group 4: Protective group rats were administrated Iβ-glucan daily as in group 3 for two weeks they induced with DEN concomitant with Iβ-glucan for six weeks. Group 5: Therapeutic group rats were induced daily with DEN then administrated with Iβ-glucan for six weeks. DEN induced liver damage as evidence by a marked increase in serum indices of liver marker enzymes (Alt, Ast and GGT), tumor markers (alpha fetoprotein) and proinflammatory cytokines(interleukin-6) when compared with the normal control group.  However, administration of irradiated β-glucan, in liver cancer induced rats displayed improvement in all measured parameters. In conclusion Iβ-glucan  considered as  a preventive and a therapeutic towards hepatocarcinogenesis via enhancing immune system in particular CD4, CD8 count and anti-inflammatory effect.
 
Keywords
Hepatocellular carcinoma; irradiated β-glucan; Tumor markers; β-catenin; caspase-3; CD4 and CD8
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