DETECTION OF GENETICALLY MODIFIED SOYBEANS DNA IN A CHEESE LIKE PRODUCT AND SOME HEAT-TREATED PRODUCTS AS FOOD MODEL | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 14, Volume 34, Issue 7, July 2009, Page 7853-7864 PDF (707.82 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2009.115395 | ||||
 View on SCiNiTO | ||||
| Author | ||||
R. M. Elsanhoty 
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| Institute of Genetic Engineering and Biotechnology, Dept. of Industrial Biotechnology, Menoufia Univ. Egypt | ||||
| Abstract | ||||
| This work was conduct to detect of recombinant DNA of genetically modified (GM) in soybean like cheese and heat-treated GM soybean milk. Genomic DNA was extracted from soybean like cheese and heat-treated GM soybeans milk by using the cetyltrimethylammonium bromide (CTAB) method and vivants DNA extraction kit. Primer pair P35s-f2 /petu-r1 amplify the junction region between CaMV35S promoter and part of CP4EPSPS and GMOScreen kit that amplify CaMV35S promoter and or NOS terminator were selected to detect the recombinant of DNA in Roundup Ready soybean. They gave PCR products of band sizes, 172 and 123 bp. PCR products were detected when DNA solution extracted from soybean like cheese and heat-treated GM soybean milk by the vivants DNA extraction kit. PCR products of the expected 172, and 123 bp were detected by agarose gel electrophoresis at the end of storage period 60 days. However, PCR products were not detected in DNA extracted from soybean like cheese and heat-treated GM soybean milk by the CTAB method. These results indicate that judicious selection of DNA extraction methods and target sequences are important to detect DNA from soybean like cheese and recombinant DNA can be detected in soybean like cheese | ||||
| Keywords | ||||
| GM soybeans; DNA extraction; processed food modal; PCR | ||||
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