Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt
Abdelaziz, I., Abd El-Tawab, A., Maarouf, A., Elhofy, F. (2020). Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt. EKB Journal Management System, 39(1), 169-174. doi: 10.21608/bvmj.2020.38777.1242
Ibtehal Saeed Abdelaziz; Ashraf Awad Abd El-Tawab; Ahmed Afifi Maarouf; Fatma Ibrahim Elhofy. "Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt". EKB Journal Management System, 39, 1, 2020, 169-174. doi: 10.21608/bvmj.2020.38777.1242
Abdelaziz, I., Abd El-Tawab, A., Maarouf, A., Elhofy, F. (2020). 'Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt', EKB Journal Management System, 39(1), pp. 169-174. doi: 10.21608/bvmj.2020.38777.1242
Abdelaziz, I., Abd El-Tawab, A., Maarouf, A., Elhofy, F. Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt. EKB Journal Management System, 2020; 39(1): 169-174. doi: 10.21608/bvmj.2020.38777.1242

Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt

Benha Veterinary Medical Journal
Article 32, Volume 39, Issue 1, September 2020, Page 169-174  XML PDF (193.8 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2020.38777.1242
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Authors
Ibtehal Saeed Abdelaziz email orcid 1; Ashraf Awad Abd El-Tawaborcid 2; Ahmed Afifi Maarouf3; Fatma Ibrahim Elhofy2
1Bacteriology. Veterinary Medicine Benha University. Egypt
2Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Benha University, Benha, Egypt
3Chief Researcher of Food Hygiene, Animal Health Research Institute, Benha Branch
Abstract
This study was conducted on 21 commercial duckling farms (1- 20 days old) inspected to show Salmonella infection in different localities at Kaliobia Governorate. Samples were taken from diseased ducklings and freshly dead for bacteriological examination which resulted in, 94 samples were positive from 630 isolates, where 28 isolates from 33 diseased ducklings and 66 isolates from 72 freshly dead ducklings. Three serogroups of Salmonella were obtained by serological identification (Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Blegdam). The antibiotic sensitivity tests for the isolated strains showed multiple antibiotic resistances (oxytetracycline; amoxicillin; ampicillin; streptomycin; erythromycin and trimethoprim/ sulphamethoxazol) but gentamycin, norfloxacin and ciprofloxacin are the most effective antibiotic on the isolated Salmonella and can be used for treatment of Salmonellosis in duck farms. PCR results appeared that, invA and stn genes were detected in all studied Salmonella isolates;pefA gene was detected in four out of five studied isolates but sefC genewas detected in two isolates only. Finally, isolated Salmonellae are virulant pathogens responsible for disease in ducklings resulting in high mortality and morbidity, gentamycin, norfloxacin and ciprofloxacin are the most proper antibiotics used for treatment of Salmonellosis in duck farms.
Keywords
Duckling; Salmonella; antibiotic resistant; virulence genes
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