Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine
El-Bagoury, G., EL-Nahas, E., Abdelazeem, L., Mohamed, A. (2018). Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine. EKB Journal Management System, 34(1), 466-473. doi: 10.21608/bvmj.2018.123434
Gabr El-Bagoury; Ehab EL-Nahas; Lila Abdelazeem; Azza Mohamed. "Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine". EKB Journal Management System, 34, 1, 2018, 466-473. doi: 10.21608/bvmj.2018.123434
El-Bagoury, G., EL-Nahas, E., Abdelazeem, L., Mohamed, A. (2018). 'Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine', EKB Journal Management System, 34(1), pp. 466-473. doi: 10.21608/bvmj.2018.123434
El-Bagoury, G., EL-Nahas, E., Abdelazeem, L., Mohamed, A. Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine. EKB Journal Management System, 2018; 34(1): 466-473. doi: 10.21608/bvmj.2018.123434

Comparative potentiality study of three different vero cell culture systems for production of PPR Vaccine

Benha Veterinary Medical Journal
Article 39, Volume 34, Issue 1, March 2018, Page 466-473  XML PDF (194.27 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2018.123434
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Authors
Gabr El-Bagoury1; Ehab EL-Nahas1; Lila Abdelazeem2; Azza Mohamed2
1Department of Virology, Faculty of Veterinary Medicine, Benha University
2Veterinary serum and vaccine Research institute, Abbasia, cairo.
Abstract
Peste des petits ruminants (PPR) is considered one of the most dangerous viral diseases of small ruminants in Africa and Asian countries. The disease control is based on vaccination of susceptible animals with an attenuated PPR virus strain (Nigeria 75/1) propagated in monolayers of Vero cells using stationery and roller flasks. This study for constructing alternative method for production of PPR vaccinal strain through propagation of PPR virus (75/l) on Vero cell culture supported on cytodex-three microcarrier beads using spinner stirring flasks and compare the recorded results with that obtained from stationary flasks (175cm2) and Roller Bottles. All cultures were propagated under the same conditions (media, pH, time of incubation) and infected with the same multiplicity of infection by PPR vaccinal virus. Samples were obtained daily for successive five days from all cultures. The PPR virus titers were 12 log10 TCID50, 5 log10 TCID50 and 6 log10 TCID50 on microcarriers culture, stationary system and roller system respectively after six days post infection. These results provide further insights to apply microcarriers cell culture technology in production of PPR vaccine.
 
Keywords
PPR; cytodex-3 microcarriers; spinner flasks; row bottles; roller bottles
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