Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation
Hassab El-Nabi, S., Khater, E., Ahmed, A., Awwad, M. (2020). Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation. EKB Journal Management System, 12(2), 111-124. doi: 10.21608/eajbsc.2020.126951
Sobhy El-Sayed Hassab El-Nabi; Eman H. S. Khater; Ahmed E. Abd El-Salam Ahmed; Mohammed Hussein Awwad. "Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation". EKB Journal Management System, 12, 2, 2020, 111-124. doi: 10.21608/eajbsc.2020.126951
Hassab El-Nabi, S., Khater, E., Ahmed, A., Awwad, M. (2020). 'Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation', EKB Journal Management System, 12(2), pp. 111-124. doi: 10.21608/eajbsc.2020.126951
Hassab El-Nabi, S., Khater, E., Ahmed, A., Awwad, M. Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation. EKB Journal Management System, 2020; 12(2): 111-124. doi: 10.21608/eajbsc.2020.126951

Study for the DNA Profiling of Mixed Samples of Multiple Contributors and Suggested Procedures to Overcome Mixture Troubleshooting in Forensic Investigation

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 9, Volume 12, Issue 2, December 2020, Page 111-124  XML PDF (1.34 MB)
Document Type: Original Article
DOI: 10.21608/eajbsc.2020.126951
View on SCiNiTO View on SCiNiTO
Authors
Sobhy El-Sayed Hassab El-Nabi1; Eman H. S. Khater2; Ahmed E. Abd El-Salam Ahmed3; Mohammed Hussein Awwad2
1Faculty of Science, Menoufia University, Shebin El-Koum, Menoufia, P. O. Box 32511
2Faculty of Science, Benha University, Benha, Qalyubia, Egypt, P.O. Box. 13518
3General Authority of Forensic Evidence, Ministry of Interior, Cairo, P.O. Box. 11517
Abstract
In modern forensic genetics, the analysis of DNA mixtures is a challenge because of the obstacles found in defining the number of contributors, especially when they exceed two. The goal of the present study was to find the most reliable method of detection for profiling the two-male and female-male mixtures obtained. Bloodstains, saliva, and body tissues from various crime scenes were obtained as samples. All samples for DNA profiling have been subjected to DNA extraction, amplification, separation, and detection. Results revealed that DNA profiling showed two-male and female-male mixtures. Results also showed the presence of a major and a minor male contributor in each genetic locus according to peak heights. Differential extraction provided a reliable method for separating female and male contributors from each other in the case of the female-male mixture. The profiling of the male donor in the female-male mixture was furtherly verified using the Y-STR profiling process. Eventually, we can infer that a good approach to identifying the two-male mixture is autosomal STR interpretation centered on major and minor differentiation. In addition, differential separation and Y-STR profiling proved to be the best processes for profiling mixed samples comprising female-male contributors as only the male profile was identified.
Keywords
DNA mixture; STR interpretation; Y-STR; differential STR profiling
Statistics
Article View: 275
PDF Download: 401