PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS
AL-GHANDOUR, A., YOUSEF, A., MOHAMED, R., TEALEB, A., AHMED, H., ATWA, H., FARAG, T. (2020). PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS. EKB Journal Management System, 50(3), 535-546. doi: 10.21608/jesp.2020.131084
ASMAA M. FAROUK AL-GHANDOUR; ASMAA MOHAMMED YOUSEF; RASHA M. S. M. MOHAMED; AL-SAYED M. TEALEB; HYTHAM K. AHMED; HANAA A. ATWA; TAHANI ISMAIL FARAG. "PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS". EKB Journal Management System, 50, 3, 2020, 535-546. doi: 10.21608/jesp.2020.131084
AL-GHANDOUR, A., YOUSEF, A., MOHAMED, R., TEALEB, A., AHMED, H., ATWA, H., FARAG, T. (2020). 'PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS', EKB Journal Management System, 50(3), pp. 535-546. doi: 10.21608/jesp.2020.131084
AL-GHANDOUR, A., YOUSEF, A., MOHAMED, R., TEALEB, A., AHMED, H., ATWA, H., FARAG, T. PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS. EKB Journal Management System, 2020; 50(3): 535-546. doi: 10.21608/jesp.2020.131084

PROPHYLACTIC ANTICRYPTOSPORIDIAL ACTIVITY OF ATORVASTATIN VERSUS NITAZOXANIDE ON EXPERIMENTALLY INFECTED IMMUNOSUPPRESSED MURINE MODELS

Journal of the Egyptian Society of Parasitology
Article 9, Volume 50, Issue 3, December 2020, Page 535-546  XML PDF (1.43 MB)
Document Type: Original Article
DOI: 10.21608/jesp.2020.131084
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Authors
ASMAA M. FAROUK AL-GHANDOUR1; ASMAA MOHAMMED YOUSEF1; RASHA M. S. M. MOHAMED2; AL-SAYED M. TEALEB3; HYTHAM K. AHMED4; HANAA A. ATWA5; TAHANI ISMAIL FARAG6
1Departments of Medical Parasitology
2Clinical Pharmacology
3Department of Pathology
4Clinical Pathology
5Pathology
6Departments of Medical Parasitology1
Abstract
This study investigated the possible prophylactic and curative role of Atorvastatin (ATV) in
treatment of cryptosporidiosis in immunosuppressed cases. Immunosuppression was done using
oral dexamethasone (0.25μg/g/day) for 14 days before infection till last scarification. The
study included 150 immunosuppressed mice in 5 major groups (N=30): G1: Normal control
group, G2: Infected control group, G3: ATV (40mg/kg/day), G4: Nitazoxanide (NTZ; 500-
mg/kg/day), G5: combination group. Each one was divided into 3 subgroups of 10 mice each:
prophylaxis ones received drug daily for 5 consecutive days before infection only, 1st and 2nd
therapeutic dose groups: mice received the drug for 1 week and 2 weeks after prophylaxis and
infection, respectively. Assessment was done parasitological by formol-ether concentration
and Modified Ziehl-Neelsen staining of stool pellets gathered weekly, immunological by serum
IFN-ɣ levels and histopathological by haematoxyline and eosin staining to determine the
drug regimens and parasite impacts on tissues.
The results showed a significant reduction in inflammatory changes of ileum, stomach and
liver histopathology and in oocysts shed on the 7th day post infection (PI) by 62.08%, 40.55%
& 71.78%, on the 14th day (PI) by 78.53%, 53.4%, 87.43% & 90.41%, 57.21%, 94.71% on
21st day (PI) in all treated groups respectively, compared to infected untreated control ones.
Sera IFN-ɣ levels showed significant increase in combination followed by ATV prophylactic
drug regimens compared to NTZ alone or infected control ones. Combined ATV and NTZ
prophylaxis gave a good synergistic anticryptosporidial efficacy in immunosuppressed mice.
Keywords
Cryptosporidium; Atorvastatin; Prophylaxis; Nitazoxanide combination; immunosuppressed
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