Long Noncoding RNA MALAT-1 and Mirna-9 Expression Profile Levels in Patients with Diabetic Polyneuropathy (DPN) and Their Correlations with The Severity of Painful DPN | ||||
| The Egyptian Journal of Hospital Medicine | ||||
| Article 22, Volume 81, Issue 6, October 2020, Page 2235-2245 PDF (502.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejhm.2020.131126 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nearmeen M. Rashad; Hala A. Fathy; Rehab M. Atef; Neveen F. Ibrahim | ||||
| Abstract | ||||
| Background: Diabetic polyneuropathy (DPN) is the major microvascular complication of type 2 diabetes mellitus (T2DM). Painful-DPN is a major cause of mortality as well as morbidity. Long non-coding RNAs (lncRNAs) and microRNAs (miRNA) have emerged as critical regulators of many diseases, however, little is known about their expression patterns and functions in T2DM and its complications. Objective: To investigate the expression profile levels of lncRNA MALAT-1 and miRNA-9 in Egyptian patients with T2DM and to explore their associations with clinical and electrophysiological tests of both painful and painless DPN. Patients and Methods: This cross-sectional controlled study enrolled 55 patients with DPN and 40 controls. All participants were subjected to a complete neurological examination and electrophysiological tests involving nerve conduction studies. The expression levels of lncRNA MALAT-1 and miRNA-9 were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Results: The relative expression levels of MALAT-1 and miRNA-9 were significantly upregulated in patients with DPN (0.219±0.061, 0.006454±0.0018, respectively) compared to controls (0.111±0.013, 0.0033±0.004, respectively). Interestingly, the relative expression levels of MALAT-1 and miRNA-9 were significantly upregulated in patients with painful DPN (0.206±0.037, 0.0045±0.0008, respectively) compared to patients with painless DPN 0.219±0.083, 0.0058±0.0017, respectively). Patients with DPN had sensory-motor axonal polyneuropathy which was affecting both lower limbs more than upper limbs. P<0.001*. Conclusions: The relative expression levels of MALAT-1 and miRNA-9 were significantly upregulated in patients with DPN more specifically in patients with painful DPN groups, hence, MALAT-1 and miRNA-9 could be used as useful and reliable diagnostic biomarkers of DPN. | ||||
| Keywords | ||||
| DPN; LNC RNA; MALAT-1; miRNA-9; Nerve conduction studies | ||||
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