Prevalence of non-O157:H7 Shiga toxin-producing E. coli in market milk at Damietta Province | ||||
| Egyptian Journal of Food Safety | ||||
| Article 17, Volume 5, Issue 1, 2018, Page 166-174 | ||||
| Document Type: Original Articles | ||||
| DOI: 10.21608/ejfsj.2018.157088 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Ashraf Nazem; Ahmed Saad; Jehan Ibrahim; Sahar Labib; Shaimaa Tony | ||||
| Abstract | ||||
| A total of one hundred raw milk samples were collected randomly from farmers, street vendors, supermarkets and dairy shops at Damietta Province. E. coli was detected in 21% of tested samples. Serological identification revealed that among the 21 isolates tested, O26: H11 was the most prevalent serotype 33.3% (7/21) while other serotypes including O91:H21, O113:H4 and O127:H6 were detected at a prevalence rates of 9.5% (2/21), 9.5% (2/21) and 14.2% (3/21), respectively. Seven other strains subdivided into O44:H18, O111:H2, O128:H2, O142, O146, O1, and O27 were detected once for each (1/21; 4.8%). Molecular characterization for detection of genes stx 1 and stx 2 indicated that such virulence genes were present in 4/5 and 5/5, 2/2 and1/2, and 1/1and1/1, in E.coli 26:H11, O91:H21 and O111:H2, respectively. The potential health hazard of the isolated strains has been discussed. | ||||
| Keywords | ||||
| Shiga-toxin; E. coli; Milk | ||||
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