The curative effect of Bee Venom and Propolis on oxidative stress induced by γ-irradiation on blood and tissues of rats | ||||
| Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology | ||||
| Article 6, Volume 6, Issue 1, June 2014, Page 53-69 PDF (515.95 K) | ||||
| DOI: 10.21608/eajbsc.2014.16047 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Abd-Elraheim A. Elshater1; Mouchira M. Mohi Eldin2; Muhammad M. A. Salman2; Naglaa R. A. Kasem2 | ||||
| 1Zoology Department, Faculty of Science; | ||||
| 2Pathology and Clinical Pathology Department, Faculty of Vet. Med., South Valley University, Qena, 83523 | ||||
| Abstract | ||||
| The biological effects of ionizing radiation result in the formation of free radicals which are the main cause of cellular damage. This study was aimed to detect the curative effect of some antioxidants drugs (Bee venom and Propolis) on the hematological, biochemical and histological parameters after exposed to γ-irradiation on the rats. Five groups of adult male albino rats were established (n= 11). The first group (Gp. 1) was served as normal group. Gp. 2 was exposed the whole body of rats to a single shot dose of gamma radiation (5Gy) and served as control group. Gp. 3 was injected intraperitoneal (i.p.) daily with Bee venomat dose (5ml/kgbody weight). Gp. 4 was given orally Propolis daily at dose 300 mg/kg body weight (B.wt). Gp. 5 was administered of (5ml/kgbody weight) of Bee venom injected i.p. plus orally 300 mg/kg (B.wt) of Propolis, daily. All groups were treated after 8 hours exposure, for 40 day. Whole blood, serum and biopsy from liver and kidneys were collected for hematological, biochemical and histopathological examinations, respectivetly. In the irradiated group (gp. 2), hematotoxicity was determined by a significant decrease in red blood cells (RBCs), white blood cells (WBCs), blood Platelets (PLTs), haemoglobin (Hb) and PCV value, as well as there are an elevation in serum ALT, AST, ALP, LDH, creatinine and urea, associated with reduction in albumin and uric acid. Hepatotoxicity was recorded in a highly significant increase malondialdehyde (MDA) with marked reduction in glutathione (GSH) levels, superoxide dismutase (SOD) and catalase (CAT) activities. In addition to, degenerative and necrotic changes showed in liver and kidneys. It could be concluded that Bee venom and Propolis, clarified a modulatory role against the cellular damage produced by free radicals induced by ionizing radiation. | ||||
| Keywords | ||||
| Bee venom; Propolis; Oxidative Stress; γ-irradiation; Rats | ||||
|
Statistics Article View: 359 PDF Download: 533 |
||||
