MOLECULAR IDENTIFICATION AND PHYLOGENTIC ANALYSIS OF POTATO LEAF ROLL VIRUS IN EGYPT | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 2, Volume 91, Issue 4, December 2013, Page 1259-1269 PDF (406.92 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2013.165097 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| HESHAM M. ABDELMAKSOUD; AYMAN M. MANDOUR; AHMED S. GAMAL ELDIN | ||||
| Plant Virology Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt. | ||||
| Abstract | ||||
| Potato leaf roll virus (PLRV) was isolated from Egyptian grown potatos. The virus was identified on the basis of host range, symptomatology, insect transmission, electron microscopy, RT-PCR, and PCR-ELISA. The complete nucleotide sequence of genomic PLRV-RNA was obtained from cloned cDNA and submitted in GenBank under Accession No. AY138970. This sequence is 5884 nucleotide long and encodes 5 ORF with an unique read-through protein suggested a conflict with the conceptual translation at amino acid 209. Comparison of PLRV sequence with that of other PLRV strains shows overall similarities of 97.02% and high genetic identity of 100% (Polish Strain), 98.5% (French Strain), 98.4% (Wageningen Strain), 98.3% (UK Strain), 98% (Canadian Strain), and 93. 5% (Australian Strain). | ||||
| Keywords | ||||
| Phylogentic analysis; PLRV similarities; Egypt PLRV | ||||
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