Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro
Theeb, B., Mohammad, F., Hashim, A., Hashim, S. (2013). Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro. EKB Journal Management System, 5(2), 35-43. doi: 10.21608/eajbsg.2013.16637
Batool Omran Theeb; Farooq Ibrahem Mohammad; Abdulkareem Jasim Hashim; Sumia Sami Hashim. "Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro". EKB Journal Management System, 5, 2, 2013, 35-43. doi: 10.21608/eajbsg.2013.16637
Theeb, B., Mohammad, F., Hashim, A., Hashim, S. (2013). 'Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro', EKB Journal Management System, 5(2), pp. 35-43. doi: 10.21608/eajbsg.2013.16637
Theeb, B., Mohammad, F., Hashim, A., Hashim, S. Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro. EKB Journal Management System, 2013; 5(2): 35-43. doi: 10.21608/eajbsg.2013.16637

Purification of hemolysin from Aspergillus fumigatus and study its cytotoxic effect on normal cell line (REF) in vitro

Egyptian Academic Journal of Biological Sciences, G. Microbiology
Article 4, Volume 5, Issue 2, December 2013, Page 35-43  XML PDF (203.36 K)
Document Type: Original Article
DOI: 10.21608/eajbsg.2013.16637
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Authors
Batool Omran Theeb1; Farooq Ibrahem Mohammad2; Abdulkareem Jasim Hashim3; Sumia Sami Hashim3
1Department of Biology, Iraqia University
2Biotechnology Research Center, Al- Nahrain University
3Department of Biotechnology, College of science, Baghdad University. Baghdad-Iraq
Abstract
Aspergillus fumigtus produced a protenaceous hemolysin, when incubated on sheeps blood agar, from 41 isolates thirty isolates (73.1%) has able to produce hemolysin. Hemolysin purified from tryptic soy broth using ion exchang and gel filtaration and found to have amolecular weight of approximately 74.52 KD a. In this study, hemolysin was used in an experimental model to study its Cytotoxic activity by evaluating effect on REF cell line (Rat embryonic fibroblast), on exposure time of  24  hrs at three different concentrations triplicate of each concentration  were used, Cytotoxicity of the purified compounds are  active against REF cell line under study and a toxic effect was clear with a significant difference at the level of probability (p< 0.05) and this effect was increase gradually with the increase of hemolysin concentration.
 
Keywords
Aspergillus fumigatus; Hemolysin; Cytotoxic activity
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