Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)
Gaafar, D., Baka, Z., Abou-Dobara, M., Shehata, H., El-Tapey, H. (2021). Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.). EKB Journal Management System, 49(1), 98-113. doi: 10.21608/ejp.2021.61270.1022
Doaa E.M. Gaafar; Zakaria A.M. Baka; Mohamed I. Abou-Dobara; Heba S. Shehata; Hany M.A. El-Tapey. "Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)". EKB Journal Management System, 49, 1, 2021, 98-113. doi: 10.21608/ejp.2021.61270.1022
Gaafar, D., Baka, Z., Abou-Dobara, M., Shehata, H., El-Tapey, H. (2021). 'Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)', EKB Journal Management System, 49(1), pp. 98-113. doi: 10.21608/ejp.2021.61270.1022
Gaafar, D., Baka, Z., Abou-Dobara, M., Shehata, H., El-Tapey, H. Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.). EKB Journal Management System, 2021; 49(1): 98-113. doi: 10.21608/ejp.2021.61270.1022

Effect of Some Microorganisms on Controlling Fusarium Wilt of Roselle (Hibiscus sabdariffa L.)

Egyptian Journal of Phytopathology
Article 8, Volume 49, Issue 1, June 2021, Page 98-113  XML PDF (811.08 K)
Document Type: Original Article
DOI: 10.21608/ejp.2021.61270.1022
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Authors
Doaa E.M. Gaafar email orcid 1; Zakaria A.M. Bakaorcid 2; Mohamed I. Abou-Dobaraorcid 2; Heba S. Shehata1; Hany M.A. El-Tapeyorcid 1
1Soils, Water and Environment Research Institute, Agricultural Research Center, 12619, Giza, Egypt.
2Botany and Microbiology Department, Faculty of Science, Damietta University, Damietta, Egypt.
Abstract
Roselle (Hibiscus sabdariffa L.) is one of the most crucial medicinal plants all over the world. The present study was carried out with the intention of solving some problems related to soil-borne diseases. Fusarium oxysporum is associated with wilt disease of roselle plant. The application of some microbiota i.e., a mixture of Pseudomonas fluorescens (PSR-11), Bacillus subtilis (BSR-8), Pleurotus ostreatus and mycorrhizeen® individually or in combination were investigated for controlling wilt disease, growth, and yield of roselle plant. The study was conducted in sandy textured soil under sprinkler irrigation of Ismailia Agricultural Research Station, Ismailia, Egypt. Under greenhouse conditions, all the tested bioagents reduced wilt incidence and increased the percentage of survived plants. The conglomeration of the bacterial mixture of Pseudomonas fluorescens and Bacillus subtilis, Pleurotus ostreatus and mycorrhizeen® was more effective in controlling wilt disease than using any of them individually. The application of the investigated bioagents showed a significant increase in phenol, photosynthetic pigments, and dehydrogenase contents, as well as root colonization percent. The obtained results showed the ability of B. subtilis and P. fluorescens to produce lytic enzymes such as β-1-3 glucanase, β-1-4 glucanase, chitinase and protease, in addition to their production of siderophore and HCN. The consortium of all microbiota gave the best results for growth and yield parameters of roselle plant.
Keywords
Roselle; Hibiscus sabdariffa; wilt; Fusarium oxysporum; biological control; Pseudomonas; Bacillus; Pleurotus; mycorrhizeen; defense-related enzymes
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