In vitro Culture of Ruta graveolens L. | ||||
Catrina: The International Journal of Environmental Sciences | ||||
Article 5, Volume 3, Issue 3, 2008, Page 33-36 PDF (657.55 K) | ||||
Document Type: Original Article | ||||
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Authors | ||||
Hamdy Attalla 1; Islam Abo El Soud2; Manal H. Eid3 | ||||
1Faculty of Agriculture, Suez Canal University. Ismailia | ||||
2Horticulture Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, Egypt | ||||
3Agricultural Botany Department, Faculty of Agriculture, Suez Canal University 41522 Ismailia, Egypt | ||||
Abstract | ||||
Shoot multiplication for Ruta graveolens L. was achieved when shoot tips excised from mature plants and cultured on Murashige and Skoog (MS) medium containing BA at 0.5, 1.0 and 2.0 mg l-1 combined with NAA at 0.1, 0.2 and 0.3 mg l-1. Highest rate of shoot proliferation was obtained after 8 weeks with the medium supplemented with BA at 0.5 mg l-1 and 0.3 mg l-1 NAA. Complete plants with strong, fibrous roots were obtained after transferring the separated shoots into rooting medium (1/2 MS or MS media with or without NAA). The survival rate of transplants during acclimatization reached 100% and the plants grew normally under the greenhouse conditions. | ||||
Keywords | ||||
micro-propagation; Ruta graveolens | ||||
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