Differential growth of Amphora coffeaeformis under different treatments of nitrogen sources. | ||||
| Egyptian Journal of Phycology | ||||
| Article 2, Volume 22, Issue 1, 2021, Page 31-47 PDF (455.85 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/egyjs.2021.90974.1005 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mahmoud A. Marzouk  1; Abo El-Khair El-Sayed2; Ahmed D. El-Gamal 3
| ||||
| 1Botany and Microbiology Department, Faculty of Science (Boys), Al-Azhar University, Cairo, Nasr City, Egypt | ||||
| 2Algal Biotechnology Unit, National Research Centre, Dokki, Cairo, Egypt | ||||
| 3Botany and Microbiology Deptment, Faculty of Science, Al-Azhar University, Cairo (Boys) | ||||
| Abstract | ||||
| The Bacillariophyta Amphora coffeaeformis C.Agardh) Kutzing was grown under indoor laboratory conditions using different nitrogen sources including magnesium nitrate ,nitric acid and urea to evaluate whether the addition of magnesium ions affect the biomass accumulation of the grown alga under this study. Concerning the effect of different nitrogen sources on Amphora growth, data revealed that 59.98 mg.L-1 of Mg(NO3)2 gave the best growth by estimating the biomass productivity of the dry weight of the alga, and the best concentration in increasing the growth rate(0.079)followed by urea then nitric acid concentrations. Different concentrations of magnesium nitrate were studied. The results showed that 50% of magnesium nitrate markedly increases the dry weight accumulation of the examined alga and surpasses control and all other treatments. On the other hand, 2 and 3 folds of magnesium concentrations (100 and 150%) resulted in the maximum productivity in which recorded 0.108 and 0.105 of growth rate, respectively. The excess of magnesium nitrate till 2 fold enhanced dry weight accumulation and 1.5 fold of magnesium nitrate resulted in the maximum (1.38g.l-1)..Scaling up was established to obtain the Amphora biomass based on the laboratory obtained data using 15x14 L photobioreactor with a final capacity of 210 L. The harvested biomass was analyzed for biochemical constituents. Daily growth parameters as dry weight (g.l-1), doubling time (g) and percentage increase (Y) were performed. Biochemical analysis of Amphora coffeaeformis biomass (%)was composed of 31.02 crude protein, 28.37 of total carbohydrates, 13.2 of oils and 12.91 of moisture under ideal laboratory conditions. | ||||
| Keywords | ||||
| Amphora coffeaeformis; magnesium source; growth; chemical composition | ||||
| References | ||||
|
Almutairi, A.W.; El-Sayed, A.B. and Reda. M.M. (2020). Combined effect of salinity and pH on lipoid content and fatty acids composition of Tisochrysis lutea. Saudi Journal of Biological Sciences, 27, 3553-3558.
Azov, Y. (1982). Effect of pH on inorganic carbon uptake in algal cultures. Appl. Environ. Microbiol., 43(6), 1300-1306.
Bhosleac, N.B.; Evansad, L.V., and Edyveanb, R.G.J. (1993). Carbohydrate production by Amphora coffeaeformis, a marine fouling diatom. Biofouling, 7(1), 81-91.
Camp, P. J. and Randall, D. D. (1985). Purification and characterization of the pea chloroplast pyruvate dehydrogenase complex. Plant Physiol., 77, 571–577.
Clément-Larosière, B.; Lopes, F.; Gonçalves, A.;Taidi, B.; Benedetti, M. and Pareau, M. M. D. (2014). Carbon dioxide biofixation by Chlorella vulgaris at different CO2 concentrations and light intensities. Eng. Life Sci., 14, 509–519.
Dinç, E. ; Ceppi, M. G.; Tóth, Z. H.;Bottka, S. and Schansker, G. (2012). The chl a fluorescence intensity is remarkably insensitive to changes in the chlorophyll content of the leaf as long as the chla/b ratio remains unaffected. Biochim. Biophys. Acta, 1817, 770–779.
Dubois, M.; Gilles,K.A.;Hamilton, J. K.; Rebers, P. A. and Smith, F, (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-356.
El-Sayed, A.B. (2010). Carotenoids accumulation in the green alga Scenedesmus sp. incubated with industrial citrate waste and different inductions stress. Nature and Science, 8(10), 34-40.
El-Sayed, A.B.; Aboulthana, W. M.; El-Feky, A.M.; Ibrahim, N.E. and Seif, M.M. (2018). Bio and phyto-chemical effect of Amphora coffeaeformis extract against hepatic injury induced by paracetamol in rats.Molecular Biology Reports, 45(10), 1-18.
El-Sayed, A.B.; Nashwa A.H. Fetyan, Fatma Ibrahim, Sayed A. Fayed, M.W. Sadik. (2020). Application of bagasse extract in economic Nannochloropsisoculata mass production. Egyptian Journal of Chemistry, 63(12), 5183 – 5192.
Fiebiger, K.; Gray, T.; Hood, M. and Hoepner, J. (2018). The Effect of Urea Concentration in Water on the Growth of Chlorella Algae. Journal of Introductory Biology Investigations, 8(1), 1-3.
Finkle, B.J. and Appleman, D. (1953).The effect of magnesium concentration on growth of Chlorella. Plant Physiol., 28, 664–673.
Gorain, P.C.; Bagchi, S.K. and Mallick, N. (2013). Effects of calcium, magnesium and sodium chloride in enhancing lipid accumulation in two green microalgae. Environmental Technology, 1-8.
Guillard, R.R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonulaconfervacea Cleve. Can. J. Microbiol., 8, 229-239.
Hildebrand, M.; Davis, A.K.; Smith, S.R.; Traller, J.C. and Abbriano, R. (2012). The place of diatoms in the biofuels industry. Biofuels, 3, 221–240.
Kumar, V.; Karela, R.P.; Korstad, J.; Kumar, S.; Srivastava, R. and Bauddh, K. (2017). Ecological,Economical and Life Cycle Assessment of Algae and Its Biofuel. In Algal Biofuels; Springer: Cham, Switzerland, 2017; pp. 451–466.
Leonardos, N. and Harris, G. N. (2006).Comparative effect of light on pigments of two strain of Emiliania huxleyi (Haptophyta), J. Phycol., 42, 1217–1224.
Ma, T.S. and C. Zauzag, 1942. Microkjeldahl determination of nitrogen. A new indicator and an improved rapid method. Ind. Eng. Chem. Ed., 14, 280.
Mehne-Jakobs, B. (1995), The influence of magnesium deficiency on carbohydrates concentrations in Norway spruce (Piceaabies) needles, Tree Physiol., 15, 577-584.
Meseck, S. and Smith, B. (2004). How high pH’s can affect the chemistry in large volume cultures of Tetraselmis chui (PLY429). J Shellfish Res., 23, 640.
Meseck, S.; Smith, B.S.; Wikfors, G.H. and Alix, J.H. (2007). Nutrient interactions between phytoplankton and bacterioplankton under different carbon dioxide regimes. Journal of Applied Phycology, 19(3), 229-237.
Morales, M., Aflalo, C. and Bernard, O. (2021). Microalgal lipids: A review of lipids potential and quantification for 95 phytoplankton species. Biomass and Bioenergy, 150, 106108.
Nelson DL and Cox MM. (2008) Lipid biosynthesis. In: Principles of biochemistry. 4th ed. New York: W. H. Freeman and Company; p. 805–845.
Rajaram, M. G.; Nagaraj, S.; Manjunath, M.; Boopathy, A.B.; Kurinjimalar, C.; Rengasamy, R.; Jayakumar, T.; Sheu, J-R. and Li, J-Y. (2018). Biofuel and Biochemical Analysis of Amphora coffeaeformis RR03, a Novel Marine Diatom, Cultivated in an Open Raceway Pond Energies, 11, 1341-1352.
Sanchez S, Martınez ME, Espinola F. (2000). Biomass production and biochemical variability of the marine microalga Isochrysis galbana in relation to culture medium, Journal of Biochemical Engineering, 6, 13–18.
Sarma, S.J.; Kumar Das,R.; Brar, S.K.; Bihan, Y.L.; Buelna, G. Verma , M. and Soccol, C.R. (2014). Application of magnesium sulfate and its nanoparticles for enhanced lipid production by mixotrophic cultivation of algae using biodiesel waste. Energy, 78, 16-22.
Scheer, H. (Ed.), Chlorophylls, CRC Press, Boca Raton, FL (1991); pp. 1–1257.
Smith, B. and Meseck, S.L. (2004). Some implications of controlling CO2 supply to cultures of Tetraselmis chui (PLY429). J. Shellfish Res., 23, 642
Sukenik, A. and Shelef, G. (1984), Algal autoflocculation-verification and proposed symbiotic Chlorella species, Phytochemistry, 31, 3103–3104.
Sunda, W.G.; Price, N.M. and Morel, F.M.M. (2005). Trace metal ion bufferes and their use in culture studies. In Algal Culturing Techniques (R.A. Andersen, ed.). Elsevier Academic Press, London, p.35-64.
Vandamme, D.; Foubert,I..;Fraeye, I..;Meesschaert,B. andMuylaert,K. (2012).Flocculation of Chlorella vulgaris induced by high pH: role of magnesium and calcium and practical implications. Bioresour. Technol., 105, 114–119.
Wright, S.W. and Jeffrey, S.W. (2005). Pigment markers for phytoplankton production, Hand book of environmental chemistry. Springer Verlag, Berlin, 71–104. | ||||
|
Statistics Article View: 647 PDF Download: 437 |
||||
