OPTIMIZATION OF FERMENTATION MEDIUM FOR α-AMYLASE PRODUCTION BY FREE AND IMMOBILIZED Aspergillus niger ON PALM TREE FIBERS | ||||
| Journal of Agricultural Chemistry and Biotechnology | ||||
| Article 2, Volume 32, Issue 8, August 2007, Page 6753-6768 PDF (712.38 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jacb.2007.201362 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Nagwa M. El-Shimi; W. A. Bazaraa; Reda M. Abou Al-Alla | ||||
| Food Science and Technology Dept. Fac. Agric. Cairo Univ. Giza, Egypt. | ||||
| Abstract | ||||
| Various strains of Aspergillus niger were screened for α- amylase synthesis. Strain 3 proved to be the highest producer (1.82 U. ml-1). Production of α- amylase by Aspergillus niger 3 in batch shaking system was optimized. The highest α- amylase production (5.63 U. ml-1) at 30 ºC was achieved when Aspergillus niger 3 was allowed to grow aerobically in a buffered medium (pH 7.0) containing 2 % dextrin and 1.6 % tryptone. The enzyme was found to be highly inducible by dextrin, while the presence of glucose or fructose repressed its synthesis. Palm tree fiber (PTF) was evaluated for the first time as a novel fungal cells supporting material. Conidiospores were firmly attached onto the rough surfaces of the PTF as proved by SEM photomicrographs. The immobilized preparations were used in batch system for α- amylase production with maximum sp. activity of 0.49 U. ml-1.mg-1. Such sp. activity was improved to 0.8 U. ml-1.mg-1 by modifying fermentation broth to contain 0.5 % dextrin and 2.4 % tryptone. | ||||
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