Determination of water soluble vitamins in Egyptian Honey by RP-HPLC

Farag, Ahmad M.; Rizk, Mahmoud S.; El-Bassel, Hamdy A.; Youssif, Mona H.; Abdel-Haleem, Fatehy M.

doi:10.21608/ajbs.2018.205550

	Determination of water soluble vitamins in Egyptian Honey by RP-HPLC
African Journal of Biological Sciences
Article 4, Volume 14, Issue 2, 2018, Page 51-61 PDF (852.38 K)
Document Type: Original Article
DOI: 10.21608/ajbs.2018.205550
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Authors
Ahmad M. Farag ¹; Mahmoud S. Rizk¹; Hamdy A. El-Bassel²; Mona H. Youssif²; Fatehy M. Abdel-Haleem¹
¹Department of Chemistry, Faculty of Science, Cairo University, Giza 12613, Egypt
²Department of Nutritional Biochemistry and Metabolism, National Nutrition Institute, Cairo, Egypt
Abstract
The purpose of this study is to evaluate water soluble vitamins (C, B₁, B₂, B₃, B₆, B₉ and B₁₂) in honey from honey bees feed on Spring flowers, Sweet marjoram, Sun flower, Clover flowers, Citrus fruits and Black seed using gradient RP-HPLC methods with PDA detector. The highest levels of vitamins C, B₁, B₂, and B₆ were observed in citrus fruits honey, sunflower honey, black seed honey and spring flower honey, respectively. The highest levels in vitamins B₃, B₉ and B₁₂ were observed in sweet marjoram honey, where the lowest levels for these vitamins were observed in clover flower honey. The lowest levels for vitamins B₂ and B₉ were observed in black seed honey. The lowest levels for vitamins C and B₁ were observed in spring flower honey and citrus fruits, respectively. These results indicated the presence of a significant difference in water soluble vitamins content depending on the type of Egyptian honey.
Keywords
HPLC; PDA; Water soluble vitamins; Egyptian honey


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