EFFECT OF ENVIROMENTAL TEMPERATURE ON ICHTHYOPHONUS HOFER/ISOLATED FROM FRESHWATER FISHES | ||||
| Egyptian Journal of Agricultural Research | ||||
| Article 25, Volume 86, Issue 3, September 2008, Page 1187-1200 PDF (3.88 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejar.2008.209347 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| AHMED F. BADRAN1; MONA M. HUSIEN2; SOAD S. A. SALAMA2 | ||||
| 1Faculty of Veterinary Medicine, Suez Canal University, Egypt | ||||
| 2Animal Health Research Institute, Agricultural Research Center, Ministry of Agriculture, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| A total of 300 fishes examined related to 3 freshwater fish species, out of which 180 samples proved to harbour Ichthyophonus hoferi(600/0) from River Nile, Ismailia Nile branch and EI.Waffa fish farm at Giza. The incidence of isolation was higher In winter season (68.75%) than in summer (45.7%). The clinical investigation revealed dark grey coloration or even blackness of the skin with deformity in the skull, and the eyes appeared doudy. The most predilection site of L holed was liver followed by spleen, heart, kidney, intestine, ovary and eyes. Post-mortem examination of Infected fishes revealed the presence of white to grey nodules of variable sizes in liver, kidney, heart, spleen and intestine. Isolation of the fungus was carried out on Sabouraud's Dextrose agar with 1% calf serum and in Minimum Essential Medium + 10% fetal calf serum at pH 3, 5, 7 and 9 within 10-15 days. At temperature 4°C the organism can still survive. The viability of the cultures was gradually diminished from 4°C to 0°C. | ||||
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