Mesenchymal Stem Cells attenuate Acetic acid-Induced Colitis in Rats via Immunomodulatory Effects | ||||
| Azhar International Journal of Pharmaceutical and Medical Sciences | ||||
| Article 10, Volume 2, Issue 1, January 2022, Page 96-107 PDF (929.19 K) | ||||
| Document Type: Original research articles | ||||
| DOI: 10.21608/aijpms.2021.79293.1079 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Hadeel A Fawzy  1; Mohammed F El-Yamany2; Hala M Fawzy1; Ebtehal M Fikry1
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| 1Department of Pharmacology, National Organization for Drug Control and Research, NODCAR, Giza, Egypt | ||||
| 2Department of Pharmacology and Toxicology, Faculty of pharmacy, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| Mesenchymal stem cells (MSCs) have recently gained popularity as a treatment for a variety of diseases. In this work, bone marrow MSCs (BM-MSCs) and adipose MSCs (AD-MSCs) were compared to dexamethasone (Dex) in the management of colitis induced by acetic acid in rats. Rats were divided in a random way into five groups: (1) Control, (2) Acetic acid treated (2 ml of 3% of acetic acid solution, intra-rectally as a single dose), (3) BM-MSCs treated (single i.p. administration of 1×106cells/rat, 24 hours after acetic acid exposure), (4) AD-MSCs treated (single i.p. administration of 1×106 cells/ rat, 24 hours after acetic acid exposure), and (5) Dex treated (0.5 mg/kg/, i.p., 1 hour after acetic acid exposure) for 7 days. Following that, after 15 days of colitis induction, all rats were decapitated. Acetic acid induced colitis was marked by elevation of edema marker, myeloperoxidase, malondialdehyde, interleukin-1β, tumor necrosis factor-alpha, interleukin-6 apoptosis via caspase-3 expression, fibrosis using Masson's trichrome stain as well as infiltration of inflammatory cells by means of hematoxylin and eosin stain, It also lowered superoxide dismutase, total antioxidant, and interleukin-10 levels. After receiving either BM-MSCs or AD-MSCs, these adverse consequences were dramatically reduced. Finally, anti-oxidant, anti-inflammatory, anti-apoptotic, and anti-fibrotic activities were detected in AD-MSCs BM-MSCs and, indicating that they could be promising novel therapeutics for the treatment of colitis. | ||||
| Keywords | ||||
| Keywords: Colitis; Mesenchymal stem cell; immunomodulation; inflammation; apoptosis, inflammation | ||||
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