Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections
Khattab, M., Taha, S., Abd El Aziz, F., Abu Shady, N., Scheuer, R., Fouchier, R., Zaki, A. (2022). Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections. EKB Journal Management System, 3(2), 279-285. doi: 10.21608/mid.2022.113594.1222
Mona Khattab; Shereen El Sayed Taha; Fatma Mohamed Abd El Aziz; Nancy Mohamed Abu Shady; Rachel Scheuer; Ron Fouchier; Ali Mohamed Zaki. "Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections". EKB Journal Management System, 3, 2, 2022, 279-285. doi: 10.21608/mid.2022.113594.1222
Khattab, M., Taha, S., Abd El Aziz, F., Abu Shady, N., Scheuer, R., Fouchier, R., Zaki, A. (2022). 'Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections', EKB Journal Management System, 3(2), pp. 279-285. doi: 10.21608/mid.2022.113594.1222
Khattab, M., Taha, S., Abd El Aziz, F., Abu Shady, N., Scheuer, R., Fouchier, R., Zaki, A. Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections. EKB Journal Management System, 2022; 3(2): 279-285. doi: 10.21608/mid.2022.113594.1222

Sequencing of Rhinoviruses in Egyptian children with respiratory tract infections

Microbes and Infectious Diseases
Article 6, Volume 3, Issue 2, May 2022, Page 279-285  XML PDF (483.36 K)
Document Type: Original Article
DOI: 10.21608/mid.2022.113594.1222
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Authors
Mona Khattab email orcid 1; Shereen El Sayed Taha1; Fatma Mohamed Abd El Aziz2; Nancy Mohamed Abu Shady3; Rachel Scheuer4; Ron Fouchier4; Ali Mohamed Zaki1
1Department of Medical Microbiology and Immunology Faculty of Medicine, Ain Shams University, Cairo, Egypt
2Department of Medical Microbiology and Immunology Faculty of Medicine, Misr University for Science and Technology, Giza, Egypt
3Department of Pediatrics Faculty of Medicine, Ain Shams University, Cairo, Egypt
4Department of Viroscience, Erasmus MC, Rotterdam, The Netherlands
Abstract
Background: Human rhinoviruses (HRV) are one of the most common causes of upper respiratory tract infections among young children.  Human rhinoviruses have  a wide genetic diversity. They include three different species A, B and-C. Acute Respiratory Infection (ARI) is considered to be  an important  cause of morbidity and mortality in neonates and children. Aim of the study:  To  detect  the common  subtypes of circulating HRV among Egyptian children with respiratory infections for  further epidemiological characterization. Methods: We enrolled 161 children admitted to Ain Shams Pediatric University Hospital complaining of respiratory tract infections.  Human rhinoviruses  were detected by RT-PCR. Sequencing of HRV was done based on viral proteins (VP4-VP2) genomic region analyses by RT-PCR. Results: HRV were detected in 54 cases (33.5%)with respiratory tract infections.Sixty-five (65) % of detected HRV was in children aged 5-10 years. Molecular sequencing  showed high prevalence of HRV-C (67%) followed by HRV-A (33%). Conclusion:  This study is from the first few studies that revealed diversity of HRV in Egypt. Different phylogenetic studies are needed to evaluate their diversity and to trace their spread and epidemiological origin.
Keywords
Rhinoviruses; Acute respiratory tract infections, RT-PCR
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