The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.
Mohammed, S., Ahmed, O., Soliman, I., Abbas, M., Mohamed, W. (2022). The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.. EKB Journal Management System, 3(2), 398-406. doi: 10.21608/mid.2022.114221.1227
Shereen Mohammed; Omnia Ashraf Ahmed; Ismail Soliman; Mostafa Samy Abbas; Wegdan Abd El Hameed Mohamed. "The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.". EKB Journal Management System, 3, 2, 2022, 398-406. doi: 10.21608/mid.2022.114221.1227
Mohammed, S., Ahmed, O., Soliman, I., Abbas, M., Mohamed, W. (2022). 'The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.', EKB Journal Management System, 3(2), pp. 398-406. doi: 10.21608/mid.2022.114221.1227
Mohammed, S., Ahmed, O., Soliman, I., Abbas, M., Mohamed, W. The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.. EKB Journal Management System, 2022; 3(2): 398-406. doi: 10.21608/mid.2022.114221.1227

The role of surfactants in inhibition of uropathogenic E. coli biofilm in catheterized patients in Assiut University Hospitals.

Microbes and Infectious Diseases
Article 19, Volume 3, Issue 2, May 2022, Page 398-406  XML PDF (505.4 K)
Document Type: Original Article
DOI: 10.21608/mid.2022.114221.1227
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Authors
Shereen Mohammed email orcid 1; Omnia Ashraf Ahmed2; Ismail Soliman3; Mostafa Samy Abbas4; Wegdan Abd El Hameed Mohamed3
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Pharmacist at Assiut General Hospital Hospital, Assiut, Egypt
3Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt
4Anesthesia and Intensive Care, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background: Escherichia coli (E. coli) represent one of the major pathogens implicated in catheter associated urinary tract infection (CAUTI). Formation of biofilm by uropathogenic E. coli (UPEC) is a major survival and persistence mechanism of bacteria against antibiotics and host immune responses in the bladder. This study was designed aiming to evaluate the role of surfactants [non-anionic: Tween 80, anionic: sodium dodecyl sulfate (SDS), cationic: cetyl trimethyl ammonium (CTMA)] on biofilm forming CsgA gene containing strains of E. coli. Methods: A cross sectional study conducted at Assiut University Hospitals ICUs included 100 patients; 53 males and 47 females, catheterized for at least 3 days. Biofilm production by E. coli isolates was detected phenotypically by culture on Congo red agar plates and confirmed genotypically by detection of CsgA by conventional PCR. Overnight broth culture of each E. coli isolate was incubated with each surfactant at 3 different concentrations (CMC). Subculture on congo red agar plates was done. Inhibition of biofilm formation was indicted by fading or absence of black color of the colonies. Results: Tween 80 showed inhibition of biofilm formation by 15% of all samples at a concentration 0.1% (>CMC). SDS show biofilm inhibition by 23% at concentration 2.32mg/ml (=CMC) and at concentration 3mg/ml (>CMC). CTMA inhibited the biofilm formation by 7% at a concentration 0.4mg/ml (=CMC) and by 38% at a concentration 0.8mg/ml (>CMC). Conclusion: Surfactants have disruptive and inhibitory effect on biofilm formation that provides an alternative for plain and medicated catheters.
Keywords
biofilm; E. coli; Curli fibers; CAUTI; surfactants
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