Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli | ||||
| Microbes and Infectious Diseases | ||||
| Article 16, Volume 3, Issue 2, May 2022, Page 366-377 PDF (544.35 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2022.118857.1242 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Aliaa Mahmoud Ghandour  1; Wegdan Abdelhamid Mohamad2; Rania Mohamed Bakry3; Asmaa Omar Ahmed4; Nahla Mohamed Elsherbiny2
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| 1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt | ||||
| 2Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut-Egypt. | ||||
| 3Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt. | ||||
| 4Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt | ||||
| Abstract | ||||
| Background:Early detection of carbapenemase enzymes among Gram negative bacilli (GNB) is mandatory to prevent their spread. Objective: The goal of this study was to evaluate the performance of chromID® CARBA-SMART medium and carbapenemase inhibition method (CIM) for detection of carbapenemases in GNB. Methodology: A total of 142 GNB isolates were collected and tested using Vitek-2® system for identification and antimicrobial susceptibility testing The carbapenem resistant (CR) GNB were tested for carbapenemase production by phenotypic methods; chromID® CARBA-SMART medium and CIM. Carbapenemase genes (𝑏𝑙𝑎NDM-1, blaSIM-1,𝑏𝑙𝑎VIM-2, 𝑏𝑙𝑎KPC-1, blaGIM-1, blaSPM-1 and 𝑏𝑙𝑎OXA-48) were detected by PCR. Results: By minimal inhibitory concentration (MIC), 111 (78.17 %) of the 142 isolates were shown to be carbapenem resistant. Sensitivity and specificity of CIM and ChromID® CARBA-SMART medium were (100% and 66.7% respectively) for CIM and (86.7% and 100% respectively) for the medium. Resistance to carbapenem was associated with high percentages of resistance to many antibiotic classes. Carbapenemase genes were detected in 82% (91/111) of CR GNB with 𝑏𝑙𝑎NDM-1 (58.6%) and 𝑏𝑙𝑎OXA-48 (55.9 %) having the highest prevalence, followed by 𝑏𝑙𝑎KPC-1 (36 %) then 𝑏𝑙𝑎VIM-2 (10.8%) and lastly blaSPM-1 (3.6 %) and blaSIM-1 (1.8 %). The blaGIM-1 gene was not detected in any isolate. Conclusion: Carbapenemase inhibition method was found to be a very sensitive, easy, and cheap test for carbapenemase detection but needs the addition of ChromID® CARBA-SMART medium to improve the specificity of the test. This study has a high prevalence of carbapenemases among isolates with potential of rapid spread necessitating need for phenotypic tests. | ||||
| Keywords | ||||
| Carbapenemase; inhibition; genes | ||||
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