GLYCEROL PRODUCTION BY OSMOPHILIC/OSMOTOLERANT MYCOBIOTA ASSOCIATED WITH MOLASSES | ||||
| Assiut University Journal of Multidisciplinary Scientific Research | ||||
| Volume 46, Issue 2, December 2017, Page 23-43 PDF (625.57 K) | ||||
| Document Type: Novel Research Articles | ||||
| DOI: 10.21608/aunj.2017.221429 | ||||
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| Abstract | ||||
| Sugarcane molasses is a by-product of sugar production from sugarcane plants. The constituents of molasses may favor the presence of osmotolerants fungi. Twenty samples of cane molasses were collected from four sugar factories from different localities in Upper Egypt during August 2015 to February 2016. Twenty-two species of osmophilic and/or osmotolerant filamentous fungi belong to 8 genera were isolated on 30% sucrose Czapek’s agar medium. The most common species were Aspergillus fumigatus, A. flavus, Monascus ruber. A total of 55 isolates belong to 22 species of the collected isolates were screened for their ability to produce extracellular glycerol using Czapek’s medium supplemented with 10% NaCl and 3% sodium bisulfite (Cz10NaCl). All isolates could produce extracellular glycerol with variable degrees (0.03-0.55 g/L). The highest producers were A. clavatus STRI 1, A. fumigatus STRI 12, A. terreus STRI 21, A. versicolor STRI 23, STR1 25 and Penicillium duclauxii STRI 45. They produced extracellular glycerol ranging between 0.51- 0.55 g/L (= 5.54 – 5.98 ml moles). On the other hand, the mycelium of both A. versicolor STRI 25 and A. clavatus STRI 1, the highest extracellular glycerol producers (5.98 and 5.87 ml moles), released respectively 63.84 and 28.90 ml moles of intracellular glycerol, when transferred into sterilized distilled water for 24 hours. | ||||
| Keywords | ||||
| Molasses; Fungi; 30% sucrose Czapek’s agar medium; Glycerol | ||||
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