IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L. | ||||
| Journal of Plant Production | ||||
| Article 10, Volume 30, Issue 12, December 2005, Page 7955-7966 PDF (174.11 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2005.237940 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| H. M. S. Aboshama | ||||
| Minufiya University -- Genetic Engineering and Biotechnology Research Institute. Plant Biotechnology Department, Egypt. | ||||
| Abstract | ||||
| Regenerating of whole plants of Calendula otticicnslis, L. was achieved through direct organogenesis using leaf and cotyledonary nodes as explants. Leaves were taken from 3·week-old in vitro plantlets and cultured on MS basal medium supplemented with 200 mg/L glutamine and 500 mgl L casein hydrolisate. The medium contained SA with different auxins (2,4-0 and NAA) Prolific direct adventitious shoot regeneration occurred on most of the tested media which that contained either 2.4-0 or NAA. The best response in terms of frequency of shoot regeneration (86.64%), number of shoots per explant (7.60) and bud forming capacity (6.58) were obtained with 1.0 mg/L 2,4-0 alone. Cotyledonary node explants were cultured on MS medium,supplemented with various concentrations of Kin and NAA. The highest frequency of regenerated shoots was achieved on MS medium supplemented with 2.0 mg/L Kin and 1.0mg/L NAA (9.20). Regenerated shoots were excised and rooted, the highest number of root/shoot was obtained with half strength MS salt medium supplemented with 1.0 mg/L ISA In vitro rooted plantlets were finally transferred to mixture of peatmoss and vermiculite al equal volume w·th survival rate95% after 21 days. Abbreviations: MS- Murashige and Skoog; 2,4-D - diChlorophenoxyacetic acid; BA-Benzyladenine ; NAA- a-naphthalene acetic acid; IBA- indole-3- butyric acid; Kin-Kinetin, BFC; Sud Forming Capacity | ||||
| Keywords | ||||
| Calendula officina/is; organogenesis; leaf culture; direct regeneration; in vitro. tissue culture; adventitious shoots; cotyledonary nodes | ||||
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