EFFECT OF MODIFICATION WHEY ENVIRONMENTAL ON WHEY PROTEIN FUNCTIONALITY AS ANTI-OXIDANT | ||||
| Journal of Food and Dairy Sciences | ||||
| Article 2, Volume 29, Issue 10, October 2004, Page 5741-5750 PDF (2.29 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jfds.2004.240569 | ||||
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| Authors | ||||
| A. S. Gad,; Hala M. Fakhr EI-Din | ||||
| Department of Dairy Science, National Research Centre, Cairo, Egypt. | ||||
| Abstract | ||||
| Whey protein is rich in cysteine residues that can be found in relatively high amounts, compared to other protein sources. Cysteine, one of three amino acids are delivered to the interior of each cell and there they are assembled into glutathione. Using whey in preparing functional diet will exposed whey protein to different environmental condition and may affect during heat processing on its stability (denaturation) that leading to loss solubility and biological functionality. In this research, many modifications for sweet whey concentrate by UF had been carried out, after that exposed to different heat treatments and study the effect of modification and heat treatment on the resultant whey components. Modifications had carried out with keeping fixed total solids (6.4%). Treatments were the original whey, concentrate sweet whey TS 8.4% and 10.5% were diluted to TS 6.4%. All samples had exposed to different heat treatments (HT), 60, 70, 80, 90 and 100·C 110 min. free lactose, whey protein nitrogen index, total and reactive sulfhydryl groups and major minerals (monovalent and divalent cations) were assayed. Results showed that, with increasing protein concentration, free lactose was decreased specially at 80·C. Modification played role in retard of denaturation as obvious from WPNI. Total and reactive sulphydryl groups has a positive correlation with protein concentrate and they increased with increasing heating, whereas divalent cations decreased with increasing HT until 80·C and rapidly increased with increasing HT to 90·C. Measurement of free sulfhydryl (SH) groups in both whey and modification whey showed that the liberation of free SH is highly correlated to the change of the heat degree. Modification high protein concentrate with phosphate buffer gave the best result for sweet whey as antioxidant potential. | ||||
| Keywords | ||||
| Whey protein; sulphydryl groups; antioxidant potential; glutathione | ||||
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