DNA POLYMORPHISM FOR EVALUATION OF FOUR PEPPER G9J- TIVARS FO~ RESISTANCE TO Meloidogyne incognita AND Rotylenchulus reniformis NEMA TOOES | ||||
| Journal of Plant Production | ||||
| Article 5, Volume 28, Issue 11, November 2003, Page 6663-6678 PDF (3.17 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.246126 | ||||
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| Authors | ||||
| A. W. Amin,1; A. A. Gharib2; Mona E. M. AI-Shalaby1 | ||||
| 1Dept 0f Agrlc. Zool. and Nematol. | ||||
| 2Dept. of Vegetable Crops, I Fac. of Agrlc., Cairo University. | ||||
| Abstract | ||||
| The reaction of four pepper cultivars (California Wonder, Long Green, Hot Green and strain-9852-17), and their plant susceptibility to inoculum relation with either Meloldogyne incognita or Rotylenchulus reniformis was studied under greenhouse conditions during 2002 and 2003 seasons. Dala showed thaI strain-9852- 17 CV. was rated as highly resistant (HR) to root-knot nematode, M. incognita and reniform nematode R. renlformis. White, California Wonder and Long Green cultivars were rated as susceptible (S) for M. incognita and R. reniformis. Whereas, Hot Green pepper cv. was rated as slightly resistant (SR) to M. incognita and R. reniformis. It was noticed that phenolic compounds had a high concentratlon in un·infected pepper than inoculated by either tested nematode. The percent age of phenol reduction was higher when the pepper infected with M. incognita than R. reniformis. On the other hand, this eVidence was conformed at the molecular level where there are the same differences batween the four tested cultivars (California Wonder, Long Green, Hot Green and slrain-9852-17) by using RAPD analysis. Amplification of genomic DNA from four pepper cultivars indicated that some bands were common to all cultivars but variations were also existing. The differences were not only in the major bands but also in some minor bands. In primer OP-MO showed total of five specifiC bands. One band can be used as a ma~er on position 950-bp in variety (strain-9852-17) that has highly resistant to M. incognita and R. reniform/so Also, in primer OP-k11 Showed total of eight specific bands. Three bands absent can be used as a marker in cullivar (strain- 9852·17) on pOSition 2SO-bp ,3QO.bp and 400-bp that have highly resistant and one band on poSition 1500-bp in one cultivar (Hot Green) which ranked as slightly resistant to both nematode specles. Whereas, in primer OP·B5 showed total of five specifIC bands can be used as a marker in one cultlvar strain-9852-17 in three position compared with those of susceptible remaining cultivars to M. incognita and R. reniformis (California Wonder and long Green) which probably have carrying a resistance gene to nematodes in this position. | ||||
| Keywords | ||||
| Genome mapping; Meloidogyne incognita; pepper cultivars; RotylenchuJus reniformis | ||||
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