MICROPROPAGATION AND SALT TOLERANCE EVALUATION IN SOME GRAPE CULTIVARS 11- SALT TOLERANCE EVALUATION: | ||||
| Journal of Plant Production | ||||
| Article 7, Volume 28, Issue 11, November 2003, Page 6833-6855 PDF (3.93 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2003.246128 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| M. B. EI-Sabrout, | ||||
| Pomology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. | ||||
| Abstract | ||||
| The present investigation was carried out during three successive years (2000· 2002), in order to evaluate three grape cultivars namely, Early Superior, Flame seedless and Thompson seedless, for salt tolerance under in vitro conditions. The main results can be summarized in the following points: A. Effect of Cultlvars 1· Early Superior gave significantly the highest shoot multiplication percentage and average number of new proliferated shoots per original shoot, in the 1'1, 2nd and 3'G subcultures, followed by Flame seedless and Thompson seedless. In the meantime, Early Superior achieved significantly the highest leaf P, K, Na, Mg, total chlorophyll contents and peroxidase activity, followed by Flame seedless and Thompson seedless. Moreover, Early Superior recorded significantly the highest leaf N and proline conlents, as compared with Flame seedless and Thompson seedless. On the contrary, Thompson seedless exhibited significantly the highest shoot injury percentage and leaf Ca content, followed by Flame seedless an1 Early Superior. Also, Flame seedless cv. exhibited Significantly the highes average length of new proliferated shoot. 2· Earty Superior produced significantly the highest rooting percentage and average number of roots per shoot, after 4 weeks of shoot culture, followed by Flame seedless and Thompson seedless. While, Early Superior and Flame seedless at the same period gave significantly the highest average root length, as compared with Thompson seedless. B.Effect of Salinity Treatments 1· In 1", 2"" and 3rG subcultures, a high decrease in shoot multiplication percentage, average number of new proliferated shoots per original shoot and average length of new proliferated shoot and a high increase in shoot injury percentage joined the increase in salinity concentrations from zero to 4000 ppm. Moreover, leaf N, Na, C\ and proline contents showed a tendency for positive responses to salinitY, treatments. On the contrary, leaf P, K, Ca, Mg. total chlorophyll contents and peroxidase activity showed a tendency for negative responses to salinity treatments. 2· After 4 weeks of shoot culture, rooting percentage, average number of roots per shoot and average root length showed a tendency for negative responses to salinity treatments, and the differences among salinity treatments were statistically significant. It is evident that, Early Superior was the most salt-tolerant cultivar, while Thompson seedless appeared to be the most satt-sensltive cultivar in this respect. ·Finally, 77%, 70% and 55% of the obtained ptants of Early Superior, Flame seedless and Thompson seedless, respectively were successfully transplanted to soil. These plants were healthy and vigorously growing under Ihe greenhouse conditions. | ||||
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