Molecular evaluation of prepared Rift Valley Fever (RVF) vaccine using Montanide Gel 02 and its effect on the genetic material | ||||
| Benha Journal of Applied Sciences | ||||
| Article 2, Volume 7, Issue 4, April 2022, Page 13-19 PDF (633.59 K) | ||||
| Document Type: Original Research Papers | ||||
| DOI: 10.21608/bjas.2022.251242 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| T A. El-Akkad1; K E. Zaki2; A G. EL Nagar3; M H. Refaat1 | ||||
| 1Department of Genetics and Genetic Engineering, Faculty of Agriculture at Moshtohor, Benha University, Egypt | ||||
| 2Serum and Vaccine Research Center, Rift Valley Department, Agriculture Research Center, Giza, Egypt | ||||
| 3Department of Animal Production, Faculty of Agriculture at Moshtohor, Benha University, Egypt | ||||
| Abstract | ||||
| Rift Valley fever (RVF) is a zoonotic, arthropod-borne viral disease that affects domestic animals. The disease is caused by the Rift Valley fever virus (Phlebovirus) that belongs to the Phenuiviridae family. Rift Valley fever (RVF) is an animal origin viral bleeding disease that affects the health of both humans and animals because it’s a Phlebovirus that makes it very dangerous. Conventional technologies were used to develop the first vaccines, such as inactivated vaccines and the live-attenuated Smithburn vaccine, using virulent RVF virus isolates. Various vaccines against the RVF virus have been developed, the modern trends in vaccine production are going to choose. The adjuvant used in the vaccine production process is a significant consideration. Therefore, in this study we explored the use of Maintained gel 0.2 as adjuvant. The proper adjuvant that can appear high and long-standing immunity. Maintained gel 0.2 is generic of maintaining as water-based adjuvant designed recently to improve efficacy of aqueous type vaccine. Therefore, we investigated the effect of inactivated RVF vaccine with three different concentrations of Montanide Gel 02 (10%, 15% and 20%). The immune response was evaluated and evaluated of the immune response in vaccinated rabbits using real time PCR (RT-PCR). Also, and in other side, starting coding target (SCOT) technique was were used to study the genetic effect of the vaccine on the rabbit’s genetic material. RT-PCR The real time PCR results for interferon γ showed that there was dramatically upregulation in transcripts levels of interferon γ with the increases of Montanide gel doses throughout the whole entire period of the experiment. A total of 10 primers were tested for selective amplification of DNA fragments. The ten SCoT primers produced reliable PCR products. However, no SCoT primers showed discernible polymorphism between all tested samples. However, no Scot primers showed discernible polymorphism between all tested samples. The study revealed that with increasing Montanide gel 02 concentration in the rabbit's vaccination, the immune response and its duration increased, with no influence on the genetic material, indicating that Montanide gel 02 was safe and effective. The immune response and its duration were increased with increasing Montanide gel 02 concentration in the rabbit's vaccination, and there was no effect on the genetic material, which gives is give an indication that Montanide gel 02 was safe and effective | ||||
| Keywords | ||||
| Rift Valley fever (RVF); Montanide gel 0.2; RT-PCR and Start Codon Targeted (SCoT) | ||||
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