Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation
Masoud, W., Sharoba, A., Osheba, A., eldesouky, A. (2022). Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation. EKB Journal Management System, 50(2), 173-189. doi: 10.21608/ejfs.2022.150252.1135
Wael Abdel-Rafie Masoud; Ashraf Sharoba; Atef Osheba; Ahmed eldesouky. "Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation". EKB Journal Management System, 50, 2, 2022, 173-189. doi: 10.21608/ejfs.2022.150252.1135
Masoud, W., Sharoba, A., Osheba, A., eldesouky, A. (2022). 'Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation', EKB Journal Management System, 50(2), pp. 173-189. doi: 10.21608/ejfs.2022.150252.1135
Masoud, W., Sharoba, A., Osheba, A., eldesouky, A. Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation. EKB Journal Management System, 2022; 50(2): 173-189. doi: 10.21608/ejfs.2022.150252.1135

Antimicrobial Activity of Nanomaterial and Essential Oil against Foodborne Pathogen on Fish Fillets during Cryopreservation

Egyptian Journal of Food Science
Volume 50, Issue 2, 2022, Page 173-189  XML PDF (1.65 MB)
Document Type: Original Article
DOI: 10.21608/ejfs.2022.150252.1135
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Authors
Wael Abdel-Rafie Masoud email orcid 1; Ashraf Sharoba2; Atef Osheba3; Ahmed eldesouky4
1Meat and Fish Technology Research Dept., Food Technology Research Institute, Agricultural Research Center, Giza, Egypt.
2Department of Food Technology,Faculty of Agriculture, Benha University, Egypt
3Vice Dean of Food Technology Research Institute for Extension and Training, Agricultural Research Center.
4Professor and Head of Food Technology, in Food Tech. Dept., Fac. of Agric., Benha Univ.
Abstract
The antibacterial activity of nanoparticles and essential oil were tested against S. typhimurium, E. coli, S. aureus and B.subtilis using agar disc diffusion assays. Essential oil and nanoparticles inhibited all tested microorganisms with different concentration against S. aureus, B. subtilis, S. typhimurium and E. coli respectively. Silver and chitosan nanoparticles showed a lower inhibition for all of the tested microorganisms compared to essential oil. The present study characterized of AgNPs (~16.77nm) and ChNPs (~20.25nm) using (TEM) and GC/MS to measure chemical components of cumin and marjoram essential oils. Gamma.-Terpinene accounts for 25.54 % of total chemical components in cumin essential oil and Bis (2-ethylhexyl) phthalate accounts for 24.48 % of total chemical components in marjoram essential oil. Fish fillets inoculated were analyzed for B. cereus and E. coli during cryopreservation. Results demonstrate that cumin essential oil at 500 ppm is more active than marjoram essential oil at 500 ppm, ChNPs 1% and AgNPs 40µg/ml respectively when compared to the control. These results revealed by increased storage time were decreased microbial growth in fish fillets inoculated with B.cereus were more active than fish fillets inoculated with E. coli. Therefore, the application of nanoparticles and essential oil has a good potential to be antimicrobial activities for various food applications.
Keywords
Antimicrobial; Nanomaterial; fish fillets
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