IMPACT OF CUl TIVAR, POLYETHYLENE GLYCOL AND TYPE OF MEDIUM ON GROWTH, DEVELOPMENT AND SOME CHEMICAL CONSTITUENTS OF MUSA SPP. CULTURED IN VITRO. | ||||
| Journal of Plant Production | ||||
| Article 6, Volume 27, Issue 9, September 2002, Page 5769-5780 PDF (2.63 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jpp.2002.256600 | ||||
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| Authors | ||||
| M. K. H. Ebrahim,1; I. A. Ibrahim2; H. A. Emara2; E. Komor3 | ||||
| 1Botany Department, Faculty of SCience, Tanta University, Tanta 31527, Egypt | ||||
| 2Genetic Engineering and Biotechnology Research Institute, Mlnufiya University, Egypt | ||||
| 3Department of Plant Physiology, Bayreuth University, 95440 Bayreuth, Germany | ||||
| Abstract | ||||
| Aseptic cultures of banana cultivars (evs) Maghraby (Mg). Valery (V). Grand Nain (GN) and Hindy (H) were estabUshed from snoot tip explants, They were initiated on M5-basal medium supplemented with 3 mgll benzyl adenine (SA) and 6 gl1 agar (starting medium). Explants were transferred at 4 week intervals until the onset of prolireration (about 2 months). Thereafter, 4 subcuttures were made on multiplication medium containing SA (5 mgJI). The produced shoots were used to evaluate lhe in vitro reseonses of tested cultivars to water (osmotic) atress induced by PEG with respect to the type at medium. Shoot survival was gradually delayed by Ihe elevated levels of PEG e 15 gJI). but the response was more pronounced in Mg and V than GN followed by H. Applying PEG (10 and 20 gl1) to the medium progressively reduced the in vitro growth and development of shoots. but the magnitude of this reduction was more evident in case ofV and Mg than GN followed by H. However, the root growth and development showed a conlrary trend under the effect of PEG, but a similar response under the effect of cultivar. AI 2 % PEG, medium liquefaction improved the growth and development of bolh shoots and roots. Moreover, the cultivar H achieved the highest values of Ihese characters, whereas V and Mg had the lowest ones. It was found that, the accumulation of lolal soluble sugars (TSS) and proline in shools was pOSItively correlated wIth waler stress induced by PEG and agar, while Ihe reverse was true in case of N. P and K accumulation. Furthermore. the metabolite accumulation (N. P, K. TSS and Proline) in shoots of PEG-treated media was superior in the cultivar H and inferior in Mg, Therefore. it was deduced that: (1) the cultivar H is the most tolerant to water stress, (2) although, Ihe cultlvars GN, V and Mg did not survive al 4.5% r;NN) PEG, the cultivar GN appeared to be more tolerant than V and Mg, and (3) the higher levels of metabolite accumulation, especially proline end TSS, enhance the plant tolerance to water stress. | ||||
| Keywords | ||||
| Osmotic stress; MicropropagatJon; Survival. Nitrogen; Phosphorus; Potassium; Sugars; Proline; Banana | ||||
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