Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model | ||||
| Egyptian Dental Journal | ||||
| Article 5, Volume 68, Issue 4 - Serial Number 3, October 2022, Page 3247-3259 PDF (2.18 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2022.135565.2092 | ||||
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| Authors | ||||
Gasser Elewa   1; Hani Abu El-ezz 2; Mahmoud Aboulfotoh 3
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| 1PhD in LASER applications in Dentistry, Oral Medicine, Diagnosis and Periodontology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt. | ||||
| 2PhD Oral Medicine and Periodontology, Oral Medicine, Diagnosis and Periodontology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt | ||||
| 3PhD Oral Biology, Oral Biology Department, Faculty of Oral and Dental Medicine, Delta University of Science and Technology, Gamasa, Egypt. | ||||
| Abstract | ||||
| Aim: This study aimed to determine the preventive and early-stage anticytotoxic effects of Ga–Al–As diode laser photobiomodulation (LPBM) and Epicatechin (green tea extract) (GT), either alone or combined, when used to treat induced oral mucositis (OM) in rats. Subjects & Methods: Thirty Wistar rats were included; two of them were sacrificed in the beginning (normal mucosa control). Radiotherapy OM was induced a fractionated course of oral radiotherapy at a rate of 2 Gy/min bilaterally twice weekly for two weeks. On day 6, four animals were sacrificed to confirm OM induction. The remaining animals (24 rats) were equally assigned into four groups: Control group (CG), Laser group (LG), green tea extract group (GG) and combined group (L+G). The continued for 5 successive days in the 3-test groups. Animal sacrifice has then occurred on day 11. The mucositis degree (MD) was clinically evaluated, the histopathological assessment of tissue changes was applied using (H&E) and Monoclonal cytochrome-c antibody. Results: All treated animals showed a statistically significant difference (P-value ≤ 0.05) than those in CG. The lased animals had a better clinical outcome with a faster healing than GT. The best clinical outcome and healing pattern were shown in (L+G). Conclusion: Ga–Al–As diode laser and Epicatechin produced protection against radiotherapy induced OM through their antioxidant and anti-apoptotic activity effects. Better outcomes were noted in favor of LPBM over GT. | ||||
| Keywords | ||||
| Oral mucositis; Laser photobiomodulation (LPBM); Epicatechin; Apoptosis | ||||
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