DETECTION OF ROTAVIRUS AND ESCHERICHIA COLI AS A CAUSE OF NEONATAL CALF DIARRHEA IN EL-WADY EL-GEDID GOVERNORATE | ||||
| Journal of the Egyptian Veterinary Medical Association | ||||
| Volume 80, Issue 2, December 2020, Page 455-476 PDF (645.23 K) | ||||
| Document Type: Original Research Articles | ||||
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| Abstract | ||||
| Rotavirus and Escherichia coli are the most common causes of scours, rotavirus alone accounts for about 27- 36% of calf scours incidence worldwide. A total of 65 fecal samples were randomly collected from diarrheic calves up 5 weeks of age in different localities in El- Wady El-Gedid governorate. All fecal samples were examined to determine the presence of bovine rotavirus (BRV) and E. coli, as causes of calf diarrhea. Twelve (18.46%) and 18 (27.69%) out of 65 fecal samples were positive to rotavirus using Lateral flow and ELISA testes respectively. The infection rate of E. coli was (46.15%).The Age with higher incidence of Rotavirus infection was recorded in 8-15 days of age (with percentage of 12.31%), but higher incidence of E. coli infection was recorded in 1-7 days of age (with percentage of 16.92%), and incidence of co-infection was (10.77%). Results also revealed that the infectious rate of BRV, E. coli and co-infection were higher in winter with percentages of 18.46 %, 24.62 % , and 7.69 % than autumn (7.69%, 12.31% and 3.08% ).In spring the infections were 1,53%, 6.15 % and zero % while in summer the rates were zero %, 3.08 % and zero % respectively. Serotyping of E. coli revealed the presence of O142, O55, O111, O27, O157, O119, O26 and O127 by a percentage of 26.67%, 20%, 13.3%, 10%, 6.6%, 3.3%, 6.6% and 6.6%, respectively. Multiplex PCR was applied for detection of the virulence genes stx1 (5/10), stx2 (3/10) and eae (6/10) in E.coli isolates | ||||
| Keywords | ||||
| Calf diarrhea; Rotavirus; ELISA; E.coli virulence and resistance genes | ||||
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