Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes
ShafiK, B., Kh., E., S., A., A., D., Ramadan, S. (2017). Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes. EKB Journal Management System, 32(1), 160-169. doi: 10.21608/bvmj.2017.31203
Basant M.N. ShafiK; El-Bayomi M. Kh.; Abo-Salem M.E. S.; Darwish S. A.; Sherif I. Ramadan. "Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes". EKB Journal Management System, 32, 1, 2017, 160-169. doi: 10.21608/bvmj.2017.31203
ShafiK, B., Kh., E., S., A., A., D., Ramadan, S. (2017). 'Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes', EKB Journal Management System, 32(1), pp. 160-169. doi: 10.21608/bvmj.2017.31203
ShafiK, B., Kh., E., S., A., A., D., Ramadan, S. Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes. EKB Journal Management System, 2017; 32(1): 160-169. doi: 10.21608/bvmj.2017.31203

Detection of SNPs in Exon10 Locus of FSHR Gene and Their Association with Some Infertility problems in Egyptian Buffaloes

Benha Veterinary Medical Journal
Article 26, Volume 32, Issue 1, March 2017, Page 160-169  XML PDF (553.89 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2017.31203
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Authors
Basant M.N. ShafiK1; El-Bayomi M. Kh.2; Abo-Salem M.E. S.2; Darwish S. A.3; Sherif I. Ramadanorcid 1
1Animal Wealth Development Dept., Fac. of Vet. Med., Benha Univ.
2Animal Wealth Development Dept., Fac. of Vet. Med., Zagazig Univ.
3Animal Production Research Institute, ARC., Ministry of Agriculture, Doki, Giza, Egypt
Abstract
Follicle-stimulating hormone (FSH) is a glycoprotein hormone. It helps in development and maturation of the
follicles in females through binding to FSH receptors which consists of 10 exons and 9 introns. The objective
of this study was to estimate some genetic factors affecting milk production and fertility traits and to detect
polymorphisms in exon 10 of FSHR gene and determine the associations between these polymorphisms and
infertility in Egyptian buffalo. Heritability estimates were (0.19, 0.18, 0.07, 0.10, 0.21 and 0.19) for calving
interval, days open, dry period, days in milk, total milk yield and 305day milk yield (305 DMY), respectively.
Dry period showed high positive phenotypic and genetic correlation with day's open and calving interval. The
breeding value for 305 DMY ranged from -480 to 380, from -370 to 330 and between -230 to 170 kg for cow,
sire and dam, respectively. There was one non synonymous SNP (A93G) at 93bp in exon 10 of FSHR gene
(with 230bp size) and there was two different pattern of single strand conformation polymorphism (SSCP) but
there was no any SNP for FSHR gene (with 306bp size) in all examined Egyptian buffaloes as shown by one
SSCP pattern and nucleotide sequencing.
Keywords
correlation. Egyptian buffaloes. FSHR. heritability. Polymorphism. SNP. SSCP
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