Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine
Hegazy, D., Warda, S., Abu Senna, A., Nagwa, S. (2016). Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine. EKB Journal Management System, 31(2), 8-15. doi: 10.21608/bvmj.2016.31253
Dalia N. Hegazy; Safaa A. Warda; Amel S. M. Abu Senna; Sidkey N. Nagwa. "Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine". EKB Journal Management System, 31, 2, 2016, 8-15. doi: 10.21608/bvmj.2016.31253
Hegazy, D., Warda, S., Abu Senna, A., Nagwa, S. (2016). 'Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine', EKB Journal Management System, 31(2), pp. 8-15. doi: 10.21608/bvmj.2016.31253
Hegazy, D., Warda, S., Abu Senna, A., Nagwa, S. Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine. EKB Journal Management System, 2016; 31(2): 8-15. doi: 10.21608/bvmj.2016.31253

Study the effect of different adjuvants on inactivated Equine herpesvirus-1 vaccine

Benha Veterinary Medical Journal
Article 2, Volume 31, Issue 2, December 2016, Page 8-15  XML PDF (368.42 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2016.31253
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Authors
Dalia N. Hegazy1; Safaa A. Warda1; Amel S. M. Abu Senna2; Sidkey N. Nagwa2
1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt
2Faculty of Science, AL-Azher university (Girls Branch)
Abstract
Equine herpesvirus-1 is a highly prevalent and frequently pathogenic infection of equines. The most serious clinical signs
of disease ranging in severity, from mild respiratory distress to abortion in pregnant mares, neonatal foal death and
neuropathogenic disorders. Inactivated EHV-1 adjuvanted vaccines consider the best way for controlling infectious
disease. In this research, the prepared EHV-1 adjuvanted vaccines was completely inactivated by binary ethyleneimine
(BEI) with (0.008M) and proved to be, sterile, pure, safe and potent when inoculated on VERO cells, mice and horses.
Challenge was applied only in inoculated mice to detect the role of all prepared vaccines in the reduction of virus clearance
and excretion. There was no any undesirable post vaccinal reaction in horses vaccinated with the prepared vaccine
reconistitued in saponin, DEAE-Dextran (100mg/dose) and adjuvanted with MontanideMTGel-01. The immunogenicity
of vaccinated mares adjuvented with different three types of vaccine was assayed by different serological tests revealed
that maximum antibodies titre was achieved at 3-4 months’ post vaccination and EHV-1 inactivated vaccine reconstituted
in DEAE-Dextran (100 mg/dose) is the best one of them followed by the vaccine reconstituted in saponin and vaccine
adjuvanted with MontanideMTGel-01
Keywords
Equine herpesvirus; inactivated vaccine; saponin; Montanide
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