Biochemical study of DNA markers for Bacterial infection in bovine mastitis | ||||
| Benha Veterinary Medical Journal | ||||
| Article 12, Volume 31, Issue 2, December 2016, Page 93-100 PDF (344.97 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31275 | ||||
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| Authors | ||||
| Afaf D. Abdel-maged1; Wael A. El Sheita2; Mohamed G. Abdelwahab3 | ||||
| 1Faculty of Veterinary Medicine, Benha University | ||||
| 2Biochemistry department, Faculty of Veterinary Medicine, Benha University | ||||
| 3Animal medicine department, Faculty of Veterinary Medicine, Benha University | ||||
| Abstract | ||||
| Mastitis is a multifactorial disease and very difficult to control. It results from injury, chemical irritation and infection caused by different bacterial species. Mastitis remains one of the most common economic problems of dairy industry worldwide as it is the most expensive disease of dairy animals resulting in the reduction of milk production and quality. These expenses in terms of reduction of production, discarding milk, drug therapy, veterinarian charges, culling of incurable animals and extrause of labor. Analysis of bacteriological examination of milk samples was made to identify the main etiological agents involved in the disease. The organisms were identified on the basis of their cultural, staining characteristics, biochemical reactions .and molecular detection. Milk sample of 23 cows, which were positive for California mastitis test, cultured for microbiological examination in the study period. Two bacterial species were isolated, Staphylococcus aureus (Staph. aureus) and E. coli bacterial isolates. The predominant isolated bacteria were Staph. aureus with isolation rate of 37.77% however E. coli was isolated with isolation rate of 13.33%). Serum alkaline phosphatase (ALP) enzyme and calcium levels were highly significant decreased while C-Reactive protein (CRP) titre and phosphorus levels were highly significant increased. Lactate dehydrogenase enzyme(LDH), Aspartate aminotransferase enzyme(AST), Gamma-glutamyl transferase enzyme(GGT), Albumin, sodium, potassium and chloride levels were nonsignificantly changed in serum of mastitic cows compared to healthy ones. While LDH, ALP and phosphorus levels were highly significant increase in milk of mastitic cows compared to that of healthy ones. However, the calcium level was high significantly decreased in mastitic cows compared to healthy ones. Molecular detection of Staph. aureus and E. coli isolates by PCR. The expected sizes of PCR products of Staph. aureus was (984bp), while that for E. coli were (662bp). | ||||
| Keywords | ||||
| Bovine Mastitis; bacteriological examination; LDH; ALP enzymes; PCR | ||||
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