Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products
Abd El Tawab, A., El-Hofy, F., Ammar, A., Aideia, H., Hammad, E. (2016). Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products. EKB Journal Management System, 31(2), 144-148. doi: 10.21608/bvmj.2016.31283
Ashraf A. Abd El Tawab; Fatma I. El-Hofy; Ahmed M. Ammar; Hoda A. Aideia; Eman A. Hammad. "Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products". EKB Journal Management System, 31, 2, 2016, 144-148. doi: 10.21608/bvmj.2016.31283
Abd El Tawab, A., El-Hofy, F., Ammar, A., Aideia, H., Hammad, E. (2016). 'Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products', EKB Journal Management System, 31(2), pp. 144-148. doi: 10.21608/bvmj.2016.31283
Abd El Tawab, A., El-Hofy, F., Ammar, A., Aideia, H., Hammad, E. Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products. EKB Journal Management System, 2016; 31(2): 144-148. doi: 10.21608/bvmj.2016.31283

Bacteriological and molecular studies on toxigenic Clostridium perfringens in milk and some milk products

Benha Veterinary Medical Journal
Article 19, Volume 31, Issue 2, December 2016, Page 144-148  XML PDF (314.42 K)
Document Type: Original Article
DOI: 10.21608/bvmj.2016.31283
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Authors
Ashraf A. Abd El Tawab1; Fatma I. El-Hofy1; Ahmed M. Ammar2; Hoda A. Aideia3; Eman A. Hammad4
1Bacteriology, Immunology and Mycology Dept., Fac. Vet. Med. Benha Univ.
2Microbiology Dept., Fac. Vet. Med. Zagazig Univ.
3Animal Health Research Institute Dokki, Giza
4General Authority for Vet. Services
Abstract
Two hundred random samples of milk, kareish cheese, yoghurt and ice-cream (50 for each) were examined
microbiologically for the presence of Clostridium perfringens, their enterotoxigencity and their antibiotic sensitivity.
Clostridium perfringens was isolated from 3 (6%) milk samples, 4 (8%) kareish cheese samples and it could not be
isolated from any examined samples of yoghurt and ice-cream. The majority of C. perfringens isolates recovered from
milk and milk products were susceptible to ofloxacin, ampicillin + sulbactam and norfloxacin (100%), vancomycin,
tetracycline, metronidazole and amoxicillin + clavulinic acid (83.3%) and clindamycin (66.7%). The majority were
resistant to cephalothin (100%), sulphamethoxazole + trimethoprim (83.3%), oxacillin and chloramphenicol (66.7%).
Molecular studies using multiplex PCR technique for detection of alpha toxin gene and C.perfringens types "A"
enterotoxin gene revealed that the 7 isolates of C. perfringens (100%) were positive for alpha toxin gene and only 2 out
of 7 isolates (28.57%) were positive for enterotoxin gene
Keywords
Milk; C. perfringens; enterotoxigencity; Antibiotic sensitivity; PCR
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