Prevalence of hard tick infesting cattle with a special reference to microscopic and molecular early diagnosis of tick born piroplasms | ||||
| Benha Veterinary Medical Journal | ||||
| Article 9, Volume 30, Issue 2, June 2016, Page 51-60 PDF (402.22 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2016.31329 | ||||
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| Authors | ||||
| Ramadan M.Y.; Elakabawy L.M.; Elmadawy R.S.; Kamal MM | ||||
| Parasitology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh,13736, Egypt | ||||
| Abstract | ||||
| The current study aimed to conclude the prevalence of hard ticks infesting cattle in Qalyobia governorate and to investigate the developmental stages of cattle piroplasms in ticks hemolymph. A total of 600 cattle were haphazardly examined for tick infestation throughout the period from April 2013 to March 2014. Hemolymph samples of 1550 ticks were extracted, stained by Giemsa again and microscopically inspected under oil immersion lens. Moreover, PCR assay was applied to amplify 18SrRNA genes of Babesia and Theileria in 40 microscopically negative hemolymph samples. The study revealed that 6.17% of cattle were infested by ticks. Rhipicephalus turanicus and Rhipicephalus praetextatus (70.02% and 29.98% respectively) were the recorded tick species. Ticks showed a great tendency to attach to the udder and inguinal regions (34.46%), while the two sides were the least infested (7.69%). No recorded significant effect of sex on the rate of infestation (P> 0.05). Cattle at age of 3-5 years were significantly more infested (78.38%) than those of 2-3 years (16.22 %) and those of 8months -2 years (5.41%). Microscopic examination of tick hemolymph revealed the presence of piroplasm developmental stages in 39.23% and 49.4% of hemolymph samples obtained from Rhipicephalus turanicus and Rhipicephalus praetextatus respectively. PCR analysis further revealed that amongst 40 ticks whose hemolymph were microscopically negative, 25% and 12.5% were positive for Babesia and Theileria sp. respectively. The results concluded that Rhipecephalus turanicus and Rhipicephalus praetextatus are the common tick species infesting cattle in the study area. PCR assay is proved to be more efficient and sensitive than the microscopic assay for identification of the developmental stages of piroplasms in tick hemolymph. Thus, allowing the easy and rapid surveillance of the endemicity of piroplasm infection among tick sp. and consequently application of emphasized control programs | ||||
| Keywords | ||||
| Tick; Babesia; Theileria; Hemolymph; Sporokinetes | ||||
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