ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT
Ali, I., Mohamed Halby, H., Salim, M., Abd-Elrady, B., Ahmed, H. (2023). ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT. EKB Journal Management System, 46(2), 1233-1248. doi: 10.21608/bfsa.2023.327640
Ibrahim Mahmoud Ali; Hamada Mohamed Mohamed Halby; Mohamed Taha Salim; Bahaa-Eldin Anwar Abd-Elrady; Heba Ahmed. "ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT". EKB Journal Management System, 46, 2, 2023, 1233-1248. doi: 10.21608/bfsa.2023.327640
Ali, I., Mohamed Halby, H., Salim, M., Abd-Elrady, B., Ahmed, H. (2023). 'ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT', EKB Journal Management System, 46(2), pp. 1233-1248. doi: 10.21608/bfsa.2023.327640
Ali, I., Mohamed Halby, H., Salim, M., Abd-Elrady, B., Ahmed, H. ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT. EKB Journal Management System, 2023; 46(2): 1233-1248. doi: 10.21608/bfsa.2023.327640

ANALYSIS OF BACTERIAL COMMUNITY AND ITS BIODEGRADATION ABILITY OF 17-Β ESTRADIOL IN WASTEWATER PLANT IN ASSIUT, UPPER EGYPT

Bulletin of Pharmaceutical Sciences Assiut University
Volume 46, Issue 2, December 2023, Page 1233-1248  XML PDF (1.2 MB)
Document Type: Original Article
DOI: 10.21608/bfsa.2023.327640
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Authors
Ibrahim Mahmoud Ali1; Hamada Mohamed Mohamed Halby2; Mohamed Taha Salim2; Bahaa-Eldin Anwar Abd-Elrady2; Heba Ahmed email orcid 3
1Department of Microbiology and Immunology, Faculty of pharmacy, Al –Azhar University, Assiut branch. Egypt
2Department of Microbiology and Immunology, Faculty of pharmacy, Al –Azhar University, Assiut branch, Egypt
3Department of microbiology and immunology, faculty of pharmacy, Minia university
Abstract
Wastewater treatment is essential for environmental sanitation in urban environments. Nevertheless, chemical, and organic substances, and microorganisms accumulate in wastewater treatment plants (WWTPs), which may be liberated into the environment via their effluents. This study aimed to analyze the bacterial community at the sewage treatment plant entrance in Assiut, Upper Egypt as well as their degradation ability of 17-β estradiol (E2) as an  Isolates were identified by conventional methods and, 16S rRNA gene sequencing. E2 degradation by different strains was assessed using HPLC/UV. Conventional methods and 16S rRNA based amplicon sequencing revealed three bacteria including Bordetella sputigena (ASc1), Pigmentiphaga daeguensis (ASc3), and Stenotrophomonas pavanii (ASc4), and when tested for E2 degradation,  they degraded 65.5%, 59%, and 66.4% of E2 (50 mg. L-1) respectively. Other bacterial strains were identified using 16S rRNA based amplicon sequencing. Bacteroidetes, Firmicutes and Proteobacteria  were the prominent phyla. However, Actinobacteria, Cyanobacteria, Chloroflexi, Caldithrix, and Chlamydiae were detected in lower proportions.
Keywords
Bacterial community composition; Wastewater treatment plants (WWTPs); 17-β estradiol (E2); Biodegradation
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