Graphene-based hydrogel silver nano-composites for antibacterial and antitumor applications: In vitro evaluation | ||||
| Journal of Scientific Research in Science | ||||
| Volume 40, Issue 1, 2023, Page 168-197 PDF (2.41 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jsrs.2023.331809 | ||||
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| Authors | ||||
Asmaa Khaled Atef  1; Tahia B. Mostafa1; Hazem M. El-Sherif2
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| 1Chemistry Department, Faculty of Women for Art, Science and Education, Ain Shams University, Héliopolis, Cairo, Egypt. | ||||
| 2Polymers and Pigments Department, National Research Centre, Cairo, Egypt. | ||||
| Abstract | ||||
| The efficiency of most cancer therapies and antibiotics is restricted due to several issues, including medication resistance and non-specific negative effects. Hydrogels based on graphene derivative and silver nano-particles (Ag-NPs) have been employed to overcome these limitations. In this manuscript, we synthesized gelatin methacryloyl (Gel-MA) to combine with functional nano-materials. Reduced graphene oxide (rGO) was synthesized by utilising ascorbic acid as a green reductant to form a composite with Gel-MA without affecting its unique characteristics. The prepared rGO and nano-composite hydrogels were characterized by the usual characteristic spectroscopic techniques. We found that the nano-composite swelled up to 2444% due to the high porosity of the hydrogel. The results demonstrated that the nanocomposite hydrogel showed higher antimicrobial activities than virgin (Gel-MA). The cytotoxicity of Gel-MA/rGO and Gel-MA/rGO/Ag composites against human lung cancer (A549) cells was evaluated using MTT assay. The Gel-MA/rGO/Ag composite showed remarkable cytotoxicity with an IC50 of 27µg/ml towards A549 cells compared with 109µg/ml of Gel-MA/rGO. | ||||
| Keywords | ||||
| Antibacterial; Antitumor; Gelatin; Gelatin methacryloyl; Hydrogel; Graphene nano-composites | ||||
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