CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS
ABD EL-KAWY, A., MOFADAL, H., YOUSEF, M., EL-SHERRY, T. (2024). CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS. EKB Journal Management System, 70(180), 151-161. doi: 10.21608/avmj.2023.242008.1196
AMANY M. ABD EL-KAWY; HAITHAM A. MOFADAL; MOHAMED SAMY YOUSEF; TAYMOUR M. EL-SHERRY. "CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS". EKB Journal Management System, 70, 180, 2024, 151-161. doi: 10.21608/avmj.2023.242008.1196
ABD EL-KAWY, A., MOFADAL, H., YOUSEF, M., EL-SHERRY, T. (2024). 'CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS', EKB Journal Management System, 70(180), pp. 151-161. doi: 10.21608/avmj.2023.242008.1196
ABD EL-KAWY, A., MOFADAL, H., YOUSEF, M., EL-SHERRY, T. CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS. EKB Journal Management System, 2024; 70(180): 151-161. doi: 10.21608/avmj.2023.242008.1196

CHARACTERIZATION OF RHEOTAXIS OF CAPACITATED AND NON-CAPACITATED BUFFALO BULLS SPERM USING MICROFLUIDICS

Assiut Veterinary Medical Journal
Article 13, Volume 70, Issue 180, January 2024, Page 151-161  XML PDF (916.74 K)
Document Type: Research article
DOI: 10.21608/avmj.2023.242008.1196
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Authors
AMANY M. ABD EL-KAWY email ; HAITHAM A. MOFADALorcid ; MOHAMED SAMY YOUSEF; TAYMOUR M. EL-SHERRY
Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt
Abstract
The present study aimed to determine the impact of sperm capacitation on sperm rheotaxis and sperm kinematics within microfluidics of buffalo cryopreserved semen. semen straws (n = 24) were obtained from 3 fertile buffalo bulls. Each straw was divided into three aliquots: control aliquot, caffeine aliquot and heparin aliquot. Sperm rheotaxis and all sperm kinematics were determined through a computer-assisted sperm analysis (CASA) system using the microfluidic platform with controlled flow velocity. The results showed that the positive rheotaxis % (PR%) of caffeine and heparin were significantly higher (PR% = 49.4±1 and 48.8±1, respectively) than control (45.1±1) (p<0.0001). Sperm kinematics show a significant variation among the three aliquots. Results show no significant differences in VCL of caffeine and heparin compared to control. While VCL of caffeine aliquot was significantly higher than that of heparin (p=0.01). The VAP of the caffeine aliquot was significantly higher than that of heparin and control (p=0.05). VSL and BCF of caffeine and heparin aliquots were significantly higher than control (p<0.001 and p<0.0001, respectively). In conclusion, this study confirms that PR% and most sperm kinematics in buffalo frozen semen improved during invitro capacitation using caffeine and heparin.
Keywords
Sperm rheotaxis; sperm kinematics; CASA; Bovine; microchannels
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