Effects of Pyrethroid Exposure on Testicular Histopathology and Biochemistry in Adult Male Wistar Rats: Evaluation of Ascorbic Acid and Alpha-Tocopherol Administration | ||||
| Journal of Advanced Pharmacy Research | ||||
| Article 4, Volume 8, Issue 1, January 2024, Page 29-37 PDF (818.28 K) | ||||
| Document Type: Research Article | ||||
| DOI: 10.21608/aprh.2023.234710.1235 | ||||
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| Authors | ||||
Temidayo Adeniyi ; Akinpelu Moronkrji  ; Daniel Eyovwerhuvu
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| Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Science Ondo-City, Ondo State, Nigeria | ||||
| Abstract | ||||
| Background: The application of pyrethroid pesticides to control pests has been widely implemented both domestically and industrially in Nigeria. Excessive and unregulated usage of pesticides can have toxic effects on the male reproductive system. Objectives: This study investigated the toxicological impact of Cypermethrin on the testes, as well as the potential mitigating effects of single and combinatorial administration of ascorbic acid and alpha-tocopherol in adult male Wistar rats. Methods: Twenty-five adult male Wistar rats weighing between 180 and 200g were randomly assigned to five groups of five rats each. Group I was the unexposed control group, while Group II was the Cypermethrin (10mg/kg/bw) exposed group. Groups III-V were exposed to Cypermethrin at standard doses of 10 mg/kg/bw and orally administered with ascorbic acid (5000mg/kg/bw), Alpha-tocopherol (3000mg/kg/bw), and coadministration of Ascorbic acid and Alpha-tocopherol respectively for 28 days. At the end of the experiment, the rats were euthanized, and their testes were harvested and processed for biochemical and histopathological analysis. Results: The findings revealed elevated malondialdehyde (MDA) levels, as well as a significant decrease in superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities (p < 0.05). There were no visible histological changes in the testes, and co-administration of ascorbic acid and alpha-tocopherol restored biochemical parameters better than single administration. Conclusion: Coadministration of ascorbic acid and alpha-tocopherol reduces cypermethrin-induced oxidative stress better than single administration, possibly due to their complementary antioxidant properties. | ||||
| Keywords | ||||
| Pesticides; pyrethroid; cypermethrin; ascorbic acid; alpha-tocopherol | ||||
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