Clinical Effect of Mesenchymal Stem cell-Derived Microvesicles as Adjunctive Treatment to Surgery on Healing of Distal Limb Chronic Wounds in Arabian Horses | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Volume 55, Issue 6, November and December 2024, Page 1765-1774 PDF (2.01 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2024.260906.1767 | ||||
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| Authors | ||||
Marwa H. Hassan1; Ahmed N. Abdallah2; Ashraf A. Shamaa3; Ashraf M. Abu-Seida   4
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| 1Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, PO: 22110, Jordan | ||||
| 2Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, Egypt | ||||
| 3Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Cairo University, Egypt. | ||||
| 4Galala University, Suez, Egypt. | ||||
| Abstract | ||||
| Distal limb wounds in horses account for more than 60% of all wounds. This study described the clinical effect of mesenchymal stem cell-derived microvesicles (MVs) on healing of equine distal limb chronic wounds. Twelve Arabian horses with distal limb granulating wounds (Group I, n=6) or ulcerated wounds (Group II, n=6) were treated surgically either by excision of the granulating masses or debridement of ulcers. In group I, the horses were divided randomly into two subgroups (3 horses each); subgroup 1 was given two doses of topical and subcutaneous injection of MVs after surgery. Subgroup 2 (control) was treated only by surgery. Group II was divided randomly into two equal subgroups; subgroup 1 was given one dose of topical and subcutaneous injection of MVs after surgery and subgroup 2 was treated by debridement (control). Healing progression was documented using digimizer image analysis software and data were statistically analyzed. The mean areas of the granulating and ulcerating wounds were 81.71±3.25 cm2 and 18.91±1.78 cm2, respectively. In group I, complete wound healing was achieved on days 70±3.5 and 100±4.32 in subgroups 1 and 2, respectively. However, complete wound healing was achieved in group II on days 27±2.3 and 41±3.1 in subgroups 1 and 2, respectively. There were statistically significant differences in wound area reduction between the two subgroups in both groups (P< .05). In conclusion, this preliminary study indicated that topical and subcutaneous injection of MVs after surgical interferences may enhance healing of the chronic wounds in distal limbs of horses. | ||||
| Keywords | ||||
| Chronic wound; Equine; Mesenchymal stem cell; Microvesicles; Regenerative medicine | ||||
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