Effect of Ammonia Stress on the Genomic DNA of Macrobrachium nipponense Collected from Al Qurna City, Iraq. | ||||
| Egyptian Journal of Aquatic Biology and Fisheries | ||||
| Article 27, Volume 28, Issue 2, March and April 2024, Page 451-458 PDF (774.82 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejabf.2024.348621 | ||||
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| Author | ||||
| Rafid M. Karim et al. | ||||
| Abstract | ||||
| Macrobrachium nipponense, decapod crustaceans belonging to Palaemonidae family, is an important nutritionally economically species. Ammonia is a major pollutant in the aquatic environment, and adversely affect the health of M. nipponense and other associated commercial species. Here, the animal of interest was exposed to acute toxicity levels of ammonia at 3, 3.5, 4, 4.5, 5, and 6mg/ L for a duration of 72 hours. DNA damage served as an indicator for ammonia toxicity in the current study. Fragmentation occurring in DNA of the animal as a result of ammonia toxicity was substantiated using the comet assay and expressed as a percentage of tail DNA. The results showed an increase in the DNA damage at ammonia concentrations of 4.5 and 5mg/ L for a duration of 72 hours, with the tail DNA percentage reaching 14 and 19%, respectively. While the highest DNA damage occurred at ammonia concentration of 6mg/ L for a duration of 72 hours, the tail DNA recorded 23%. These findings suggested that exposure to ammonia caused damage in the DNA of M nipponense which reflects its effect on the physiological functions and threaten this economically important animal. | ||||
| Keywords | ||||
| Macrobrachium nipponense; Comet assay; Ammonia | ||||
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