The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria
Kamel, D., Osman, D., Gomah, N., Hassan, A. (2018). The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria. EKB Journal Management System, 9(3), 121-126. doi: 10.21608/jfds.2018.35427
Dalia Kamel; Dina Osman; Nanis Gomah; A. Hassan. "The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria". EKB Journal Management System, 9, 3, 2018, 121-126. doi: 10.21608/jfds.2018.35427
Kamel, D., Osman, D., Gomah, N., Hassan, A. (2018). 'The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria', EKB Journal Management System, 9(3), pp. 121-126. doi: 10.21608/jfds.2018.35427
Kamel, D., Osman, D., Gomah, N., Hassan, A. The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria. EKB Journal Management System, 2018; 9(3): 121-126. doi: 10.21608/jfds.2018.35427

The Combined Effect of Fortification of Media with Peptone and Control of Acidity on the Growth of Lactic Acid Bacteria

Journal of Food and Dairy Sciences
Article 5, Volume 9, Issue 3, March 2018, Page 121-126  XML PDF (425.92 K)
Document Type: Original Article
DOI: 10.21608/jfds.2018.35427
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Authors
Dalia Kamel; Dina Osman email ; Nanis Gomah; A. Hassan
Department of Dairy Science, Faculty of Agriculture, Assiut University.
Abstract
The present work was carried out to investigate the combined effect of fortification of media with 1% peptone under control of culture acidity on the growth rate of Lactococcus lactis subsplactis , Lactococcus lactis subsp cremoris and Lactobacillus casei in sterilized skim milk incubated at 34°C. The obtained results indicated that:Lactococcus lactis subsp lactis grow in sterilized skim milk resulted in coagulation of milk after 12th hour of incubation, and was reached plate count of 28x106 CFU/ml.When the same strain grown in sterilized milk supplemented with 1% peptone under  control of culture acidity no coagulation was observed up to the end of incubation period after 28th hour at 34°C, and it reached a plate count of 70x106 CFU/ml at the end of the exponential phase after 12th hour of incubation Cessation of growth in the culture started the stationary phase after 24th hour of incubation. The effect of added 1% peptone to sterilized skim milk under control of culture acidity on the colony forming units of Lactococcus lactis subsp cremoris, indicated that control sample grown in sterilized skim milk reached count of 55x106 CFU/ml after 12th hour of incubation. The corresponding values for Lactococcus lactis subsp cremoris grow with medium supplementation and control of culture acidity reached a plate count of 20x107 CFU/ml after 24th hour of incubation.When Lactobacillus casei grow in sterilized skim milk incubated at 34°C with medium supplementation with 1% peptone under control of culture acidity, the population density was several times higher in culture supplemented with peptone under control of culture acidity reached a population density of 17x107 CFU/ml, while only 23x106 CFU/ml were recorded by the same strain being grown as a control after 24th hour of incubation.
Keywords
lactic acid bacteria; titratable acidity; colony count; culture acidity control; Mrs Media
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